Evidence map›Paper›PMID 41734060›Full record

ArticleSTAR protocols2026

Protocol for analyzing immune cell infiltration in 3D pancreatic heterotypic spheroids composed of tumor cells and cancer-associated fibroblasts.

Bessede Thomas, Vezzio-Vie Nadia, Freixinos Clara, Pirot Nelly, Bonnefoy Nathalie, Gongora Celine, Larbouret Christel, Gros Laurent

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Bessede ThomasIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Vezzio-Vie NadiaIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Freixinos ClaraIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Pirot NellyIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Bonnefoy NathalieIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Gongora CelineIRCM, University Montpellier, Inserm, ICM, Montpellier, France.
Larbouret ChristelIRCM, University Montpellier, Inserm, ICM, Montpellier, France. Electronic address: christel.larbouret@inserm.fr.
Gros LaurentIRCM, University Montpellier, Inserm, ICM, Montpellier, France. Electronic address: laurent.gros@inserm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a protocol to generate and analyze 3D cancer models composed of tumor cells, cancer-associated fibroblasts (CAFs), and immune cells that mimic the complexity of the pancreatic ductal adenocarcinoma (PDAC) stroma. The protocol includes steps and conditions for establishing 3D co-cultures, embedding for histological analysis, and flow cytometry analysis for the immunophenotyping and characterization of tumor-infiltrating immune cells. These models are valuable for testing strategies and studying the roles of CAFs and immune cells in tumor growth, chemoresistance, and immunosuppression.

Indexed as

Cancer-Associated FibroblastsCarcinoma, Pancreatic DuctalPancreatic NeoplasmsSpheroids, CellularCell Line, TumorCoculture TechniquesFlow CytometryHumansCancerCell BiologyImmunology

Identifiers

PMID41734060
PMCPMC13080457

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.