Evidence map›Paper›PMID 41735679›Full record

ArticleNature metabolism2026

PFKM governs metabolic shifts throughout skeletal muscle differentiation.

Melissa Campos, Steven T Nguyen, Xiangduo Kong, Ying Yang, Richard L Watson, Anastasia Gromova, Catherine R Livelo, Carolina N Franco, Julia E Cabral, Laurence J Seabrook and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature metabolism, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Melissa CamposDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.
Steven T NguyenDepartment of Pharmaceutical Sciences, School of Pharmacology and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
Xiangduo KongDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA, USA.
Ying YangDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.
Richard L WatsonDepartment of Biological Chemistry, School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Anastasia GromovaDepartment of Pathology and Laboratory Medicine, School of Medicine, University of California, Irvine, Irvine, CA, USA.
Catherine R LiveloDepartment of Pharmaceutical Sciences, School of Pharmacology and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
Carolina N FrancoDepartment of Pharmaceutical Sciences, School of Pharmacology and Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
Julia E CabralDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0001-6072-6362
Laurence J SeabrookDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0002-9337-9712
Shengqi DaiDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.
Yingzi LiuDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.
Mingqi ZhouDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA, USA.
Eric A HanseDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0002-1234-0198
Kaelyn SumigrayDepartment of Genetics, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID http://orcid.org/0000-0002-1267-7559
Albert R La SpadaDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA, USA.
Marcus M SeldinDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0001-8026-4759
Maksim V PlikusDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0002-8845-2559
Dequina A NicholasDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0003-4996-2190
Reginald McNultyDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0003-2101-1377
Mei KongDepartment of Molecular Biology and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA.ORCID http://orcid.org/0000-0001-8139-2349
Kyoko YokomoriDepartment of Biological Chemistry, School of Medicine, University of California, Irvine, Irvine, CA, USA.
Lauren V AlbrechtDepartment of Developmental and Cell Biology, Charlie Dunlop School of Biological Sciences, University of California, Irvine, Irvine, CA, USA. lvalbrec@uci.edu.ORCID http://orcid.org/0000-0002-3147-8010

Funding

Genetic and epigenetic mechanisms of FSHD pathogenesisR01AR071287 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Seyed Ali Mortazavi, Kyoko Yokomori · 2017 to 2026
$4.6M
Deciphering Novel Principles of Rapid Proteostatic Control and Innovating Spatiotemporal Lysosomal ToolsR35GM157370 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Lauren Veronica Albrecht · 2024 to 2026
$1.1M
MAXIMUS: MAXimizing Training in MUScle ResearchT32AR083870 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI TAHSEEN MOZAFFAR, Sergio Armando Villalta · 2024 to 2026
$577k
Alveolar macrophage and epithelial cell lipid metabolism in pulmonary fibrosisK08HL179476 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Richard L. Watson · 2025 to 2026
$340k
NHLBI NIH HHS K08 HL179476NIAMS NIH HHS R01 AR071287NIAMS NIH HHS T32 AR083870NIGMS NIH HHS R35 GM157370
6 · The paper itself

Abstract

Metabolism is known to influence cell identity, but the underlying mechanisms remain unclear. Here we reveal spatiotemporal dynamics of phosphofructokinase 1 (PFK1), a key glycolytic enzyme, within the skeletal muscle lineage. The expression of PFKM (the muscle isoform of PFK1) is low in muscle stem cells and increases during differentiation. Mechanistically, Wnt signalling rapidly induces lysosomal degradation of PFKM through a methyl arginine degron motif, which gets selectively methylated by the protein arginine methyltransferase (PRMT1) and delivered to lysosomes through microautophagy. PFKM degradation shifts glucose metabolism from glycolysis to the pentose phosphate pathway. PFKM overexpression increases glycolysis and promotes differentiation into terminally differentiated myofibres. On the other hand, PFKM knockdown blunts differentiation, which can be rescued by supplementation with the downstream glycolytic intermediate 3-phosphoglycerate. In sum, our findings highlight the importance of compartmentalized metabolism in cell fate decisions.

Indexed as

Cell DifferentiationMuscle, SkeletalPhosphofructokinase-1AnimalsGlycolysisLysosomesMiceWnt Signaling PathwayPhosphofructokinase-1

Identifiers

PMID41735679
PMCPMC12945692

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.