Evidence map›Paper›PMID 41737224›Full record

ArticleFrontiers in immunology2026

Integrated single-cell and spatial transcriptomics reveal the differentiation drivers of gastric epithelial lineage progression.

Xuyu Chen, Xin Jiang, Siying Wang, Jianlei Xia, Wenjun Wang, Xinyu Fu, Ping Yukun, Zhuoqi Liu, Yaoyao Li, Min Zhang and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Xuyu Chen *Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Xin Jiang *Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Siying Wang *Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Jianlei Xia *Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Wenjun WangDepartment of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Xinyu FuDalian Medical University, Dalian, Liaoning, China.
Ping YukunDepartment of Neurology, Northern Jiangsu People's Hospital, Yangzhou, China.
Zhuoqi LiuDepartment of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Yaoyao LiDepartment of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Min ZhangDepartment of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.
Yanbing DingDepartment of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastric cancer (GC) develops through a sequence from chronic gastritis to intestinal metaplasia (IM) and carcinoma, with Helicobacter pylori (HP) as a key driver; however, the molecular mediators linking inflammation to malignant transformation remain unclear. We integrated single-cell RNA sequencing and spatial transcriptomics of gastric mucosal samples from atrophic gastritis, IM, and GC, including HP positive (+) and HP negative (-) cases, to map cellular heterogeneity, differentiation trajectories, and pathway activities. Our analyses revealed that IM epithelium represents a transitional state between normal and malignant epithelial lineages, characterized by enhanced WNT signaling that promotes neoplastic progression, whereas H. pylori-associated inflammation activates NF-κB signaling. Across analysis, UPP1 was consistently upregulated in malignant and H. pylori-positive epithelium, increasing along pseudotime toward cancer-like states. Spatial mapping and organoid experiments confirmed that UPP1-high cells had higher intestinal differentiation scores, while UPP1 knockout promoted IM-like morphology in WNT-depleted cultures. Clinically, UPP1 was elevated in GC versus normal tissues, correlated with advanced TNM stage, predicted poor survival, and was higher in HP

Indexed as

Epithelial CellsGastric MucosaStomach NeoplasmsCell DifferentiationCell LineageCell Transformation, NeoplasticDisease ProgressionGastritis, AtrophicGene Expression ProfilingHelicobacter InfectionsHelicobacter pyloriHumansMetaplasiaSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisSpatial Transcriptomicsepithelial differentiationgastric cancerHelicobacter pyloriintestinal metaplasiaUPP1

Identifiers

PMID41737224
PMCPMC12926446

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.