Evidence map›Paper›PMID 41739316›Full record

ArticleCell biochemistry and biophysics2026

Polyherbal Formulation Attenuates Isoproterenol-Induced Cardiac Toxicity Through Regulation of Mitochondrial Bioenergetics, Lipid Toxicity, and Intrinsic Apoptotic Pathways in Rats.

V V Sathibabu Uddandrao, S Nivetha, P Chandrasekaran, S Sengottuvelu, Brahma Naidu Parim, Umesh Kumar, Andamuthu Yamunadevi, Kaushik Das

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Article in Cell biochemistry and biophysics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

V V Sathibabu UddandraoDepartment of Biotechnology, Karpagam Academy of Higher Education (Deemed to be University), Coimbatore, 641021, Tamil Nadu, India. sathibabuuddandrao.veeraraghavulu@kahedu.edu.in.
S NivethaDepartment of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637215, Tamil Nadu, India.
P ChandrasekaranDepartment of Biochemistry, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637215, Tamil Nadu, India.
S SengottuveluDepartment of Pharmacology, Nandha College of Pharmacy, Erode, 638052, Tamilnadu, India.
Brahma Naidu ParimAnimal Physiology and Biochemistry Laboratory, ICMR-National Animal Resource Facility for Biomedical Research (ICMR-NARFBR), Hyderabad, 500078, India.
Umesh KumarUniversity Institute of Engineering, Chandigarh University, NH5, Gharuan, Chandigarh-Ludhiana Highway, Mohali, Punjab, India.
Andamuthu YamunadeviDepartment of Oral Pathology and Microbiology, Nandha Dental College and Hospital, Erode, 638052, Tamil Nadu, India.
Kaushik DasBiotechnology Research and Innovation Council (BRIC)-National Institute of Biomedical Genomics (NIBMG), Kalyani, Nadia, 741251, West Bengal, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isoproterenol (ISO) is a synthetic β-adrenergic agonist widely used to model xenobiotic-induced cardiac injury, primarily through mechanisms involving oxidative stress, mitochondrial dysfunction, dyslipidemia, and apoptosis. Considering the growing interest in plant-derived bioactive compounds as cardioprotective agents, this study evaluated the mechanistic potential of a polyherbal formulation (PHF) composed of Andrographis paniculata, Bauhinia variegata, Moringa oleifera, and Lantana camara. GC-MS profiling of the formulation revealed several pharmacologically relevant phytoconstituents, including 3-methylmannoside, isothiourea, L-gala-L-ido-octose, β-sitosterol, heptanal, and eicosatrienoic acid methyl ester. ISO challenge in rats resulted in pronounced cardiac injury marked by elevated biomarkers, perturbed lipid metabolism, depletion of endogenous antioxidants, mitochondrial ATP loss, Ca2+ overload, suppression of tricarboxylic acid cycle enzymes, and activation of Bax/caspase-3-mediated apoptosis. Pretreatment with PHF significantly attenuated these pathological changes by restoring antioxidant defenses, stabilizing lipid homeostasis, improving mitochondrial bioenergetics, enhancing TCA cycle activity, and modulating intrinsic apoptotic signalling. Additionally, PHF upregulated mitochondrial biogenesis mediators (PGC-1α, NRF1, TFAM) and promoted AMPK-PPARα-driven fatty acid oxidation while suppressing SREBP-1c-associated lipogenesis. Molecular docking further supported the multi-target nature of the phytochemicals through strong interactions with mitochondrial, lipid-regulatory, and apoptotic proteins. Collectively, these findings highlight the pharmacognostic value of PHF and demonstrate its potential as a natural cardioprotective intervention capable of modulating key metabolic and mitochondrial pathways involved in xenobiotic-induced cardiac toxicity.

Indexed as

ApoptosisEnergy MetabolismIsoproterenolLipid MetabolismMitochondriaMitochondria, HeartPlant ExtractsAnimalsCardiotonic AgentsCardiotoxicityMaleMolecular Docking SimulationOxidative StressRatsCardiotonic AgentsIsoproterenolPlant ExtractsApoptosisCardiac toxicityIsoproterenolLipotoxicityMitochondrial dysfunctionPolyherbal formulation

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.