Protocol for KAT8 conditional knockout mice generation and its application in 4NQO-induced esophageal tumor model.

Dandan Zhang, Ming Jiang, Kangdong Liu, Zigang Dong

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Dandan ZhangChina-US (Henan) Hormel Cancer Institute, No. 127, Zhengzhou, Henan 450000, China; Institute of Trauma and Metabolism, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, China.

Ming JiangChina-US (Henan) Hormel Cancer Institute, No. 127, Zhengzhou, Henan 450000, China.

Kangdong LiuChina-US (Henan) Hormel Cancer Institute, No. 127, Zhengzhou, Henan 450000, China; Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450000, China; The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou 450000, China; State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou, Henan 450000, China. Electronic address: kdliu@zzu.edu.cn.

Zigang DongChina-US (Henan) Hormel Cancer Institute, No. 127, Zhengzhou, Henan 450000, China; Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450000, China; The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou 450000, China; State Key Laboratory of Esophageal Cancer Prevention and Treatment, Zhengzhou University, Zhengzhou, Henan 450000, China. Electronic address: dongzg@zzu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

4-Nitroquinoline 1-oxide (4NQO), a quinoline derivative, induces tumor formation in mice following a multi-stage pattern analogous to human tumors, mimicking the natural carcinogenesis process. Here, we present a protocol for inducing esophageal squamous cell carcinoma (ESCC) in mice using 4NQO. We detail the acquisition of KAT8 esophagus-specific knockout mice, the establishment of 4NQO-induced ESCC, and the validation of the model via western blotting (WB), hematoxylin and eosin (H&E) staining, and immunohistochemistry (IHC). For complete details on the use and execution of this protocol, please refer to Zhang et al.

Indexed as

4-Nitroquinoline-1-oxideEsophageal NeoplasmsEsophageal Squamous Cell CarcinomaHistone AcetyltransferasesAnimalsDisease Models, AnimalMiceMice, Knockout4-Nitroquinoline-1-oxideHistone AcetyltransferasesBiotechnology and bioengineeringCancerCRISPRModel Organisms

Identifiers

PMID41739644

DOI10.1016/j.xpro.2026.104384

PMCPMC12950344

What Socratic holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.