Evidence map›Paper›PMID 41742040›Full record

ArticleBMC molecular and cell biology2026

ATP-related purinergic signaling via P2Y12 contributes to airway epithelial barrier integrity.

Kota Tsuya, Shuichiro Maruoka, Shinichi Okamoto, Sotaro Shikano, Mari Ujike, Shiho Yamada, Yusuke Kurosawa, Kenji Mizumura, Yutaka Kozu, Yasuhiro Gon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kota Tsuya *Division of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Shuichiro Maruoka *Division of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Shinichi OkamotoDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Sotaro ShikanoDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Mari UjikeDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Shiho YamadaDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Yusuke KurosawaDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Kenji MizumuraDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.
Yutaka KozuDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan. kozu.yutaka@nihon-u.ac.jp.
Yasuhiro GonDivision of Respiratory Medicine, Department of Internal Medicine, Nihon University School of Medicine, 30-1 Oyaguchi-Kamicho, Itabashi-ku, Tokyo, 173-8610, Japan.

Funding

Grants-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Nihon University Multidisciplinary Research Grant for 2021 18K07482 and 21K07400Strategic Research Base Development Program for Private Universities, subsidized by MEXT S1511014
6 · The paper itself

Abstract

backgroundThe airway epithelial barrier, which defends against external pathogens, is compromised in bronchial asthma. This study investigated the roles of adenosine triphosphate (ATP) and specific purinergic receptors in airway epithelial barrier function. Using the 16HBE14o- human airway epithelial cell line, we measured barrier integrity using transepithelial electrical resistance and the apparent paracellular permeability coefficient.

resultsATP enhanced barrier integrity in a dose-dependent manner without affecting cell proliferation. Real-time PCR revealed an increased expression of tight and adherens junction proteins, including E-cadherin and occludin, following ATP stimulation. Our findings suggest that P2Y12 contributes to ATP-related purinergic signaling associated with enhanced airway epithelial barrier integrity.

conclusionsThese findings suggest that ATP-related purinergic signaling via P2Y12 may contribute to the regulation of airway epithelial barrier integrity in vitro. Further studies in more physiologically relevant systems are warranted to determine the clinical relevance of these observations.

Indexed as

Adenosine TriphosphateEpithelial CellsReceptors, Purinergic P2Y12Respiratory MucosaSignal TransductionAdherens JunctionsCadherinsCell LineHumansOccludinPermeabilityTight JunctionsAdenosine TriphosphateCadherinsOccludinReceptors, Purinergic P2Y12Adherens junctionsAirway epithelial barrierATPBronchial asthmaP2Y12Purinergic receptorsTight junctions

Identifiers

PMID41742040
PMCPMC13041268

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.