Evidence map›Paper›PMID 41744827›Full record

ArticleCells2026

Transcriptomic Analysis of Adult Mouse Cardiac Stromal Cells Using Single-Cell qRT-PCR.

Rita Alonaizan, Patricia Chaves-Guerrero, Sara Samari, Michela Noseda, Nicola Smart, Carolyn Carr

Abstract read
In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rita AlonaizanDepartment of Physiology, Anatomy & Genetics, University of Oxford, Oxford OX1 3PT, UK.ORCID 0000-0003-1118-5015
Patricia Chaves-GuerreroNational Heart and Lung Institute, Imperial College London, London W12 0NN, UK.ORCID 0000-0003-1364-0130
Sara SamariNational Heart and Lung Institute, Imperial College London, London W12 0NN, UK.
Michela NosedaNational Heart and Lung Institute, Imperial College London, London W12 0NN, UK.
Nicola SmartDepartment of Physiology, Anatomy & Genetics, University of Oxford, Oxford OX1 3PT, UK.ORCID 0000-0002-3501-7254
Carolyn CarrDepartment of Physiology, Anatomy & Genetics, University of Oxford, Oxford OX1 3PT, UK.ORCID 0000-0003-0102-5261

Funding

King Faisal Specialist Hospital & Research Centre This research was funded by PhD scholarship to R.A.
6 · The paper itself

Abstract

Fate-mapping studies have challenged the longstanding view of the adult mammalian heart as a post-mitotic organ, suggesting limited cardiomyocyte renewal. This has spurred efforts to determine whether selected cardiac stromal cells have regenerative potential; however, their contribution to cardiac regeneration has been found to be minimal compared with that of cardiomyocyte proliferation. Despite this, transplantation of some cardiac stromal cell populations has shown therapeutic potential through paracrine signalling. The identity of the paracrine-active stromal cell populations remains unclear due to overlapping characteristics with other cardiac stromal cell populations, such as fibroblasts, mesenchymal cells, and pericytes. This study sought to clarify the transcriptional identity and heterogeneity of adult mouse cardiac stromal cells by developing a cardiac collagenase-trypsin protocol and comparing it to the established method for isolating cardiosphere-derived cells (CDCs). This novel protocol resulted in a higher cell yield and shorter expansion time, and the resulting cells showed superior survival under serum starvation compared to commercially acquired cardiac fibroblasts (CFs). Single-cell qRT-PCR analysis revealed that collagenase-trypsin cells (CTs) and CDCs share similar gene expression profiles, distinct from those of CFs. Notably, CTs exhibited higher expression of

Indexed as

Gene Expression ProfilingMyocardiumReal-Time Polymerase Chain ReactionSingle-Cell AnalysisStromal CellsTranscriptomeAnimalsBasic Helix-Loop-Helix ProteinsCollagenasesFibroblastsMiceMice, Inbred C57BLMyocytes, CardiacSingle-Cell Gene Expression AnalysisTrypsinBasic Helix-Loop-Helix ProteinsCollagenasesTcf21 protein, mouseTrypsincardiac paracrine-active stromal cellscardiosphere-derived cellscell therapyheart regenerationmyocardial infarctionsingle-cell qRT-PCR

Identifiers

PMID41744827
PMCPMC12939824

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.