Evidence mapPaperPMID 41745567Full record

ArticleJournal of functional biomaterials2026

Oxidative Stress, Pro-Inflammatory Response, Cytotoxicity and Apoptosis Induced by Contemporary Endodontic Sealers in Human Periodontal Ligament Fibroblasts.

Stanisław Krokosz, Virginia Ewa Lis, Sara Zięba, Mateusz Maciejczyk, Ewa Zalewska, Maria Obrycka, Edyta Gołaś, Małgorzata Żendzian-Piotrowska, Jerzy Ładny, Anna Skutnik-Radziszewska and 3 more

Abstract read
In one paragraph

Article in Journal of functional biomaterials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Stanisław KrokoszDepartment of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.ORCID 0009-0006-8271-6556
Virginia Ewa LisDepartment of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.ORCID 0009-0004-1987-6261
Sara ZiębaIndependent Researcher, 15-089 Białystok, Poland.
Mateusz MaciejczykDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 15-233 Białystok, Poland.ORCID 0000-0001-5609-3187
Ewa ZalewskaStudents' Research Group at the Department of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.
Maria ObryckaDoctoral School, Medical University of Bialystok, 15-089 Białystok, Poland.ORCID 0009-0001-1581-2154
Edyta GołaśDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 15-233 Białystok, Poland.
Małgorzata Żendzian-PiotrowskaDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 15-233 Białystok, Poland.ORCID 0000-0002-4350-0369
Jerzy ŁadnyDepartment of Emergency Medicine, Medical University of Bialystok, 15-089 Białystok, Poland.
Anna Skutnik-RadziszewskaDepartment of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.ORCID 0000-0003-3676-1488
Karol DąbrowskiDepartment of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.
Julia KuźmiukIndependent Public Health Care Complex in Minsk Mazowiecki, 05-300 Minsk-Mazowiecki, Poland.
Anna ZalewskaDepartment of Restorative Dentistry, Medical University of Bialystok, 15-089 Białystok, Poland.ORCID 0000-0003-4562-0951

Funding

Medical University of Białystok B.SUB.25.384
6 · The paper itself

Abstract

The biological compatibility of endodontic sealers is a key determinant of periapical tissue healing. This in vitro study investigated the cytotoxic, pro-inflammatory, and redox-related effects of eight endodontic sealers on human periodontal ligament fibroblasts (HPdLFs): Biopulp (Chema-Elektromet), AH Plus (Dentsply Sirona), MTA Fillapex (Angelus), EndoSeal MTA (Maruchi), GuttaFlow (Coltène), AH Plus Bioceramic (Dentsply Sirona), TotalFill BC (FKG Dentaire SA), and BioRoot TM (Septodont). Cells were exposed for 24 h to 10-fold-diluted sealer extracts prepared in accordance with the manufacturers' instructions, while control samples underwent identical procedures without sealer contact. Oxidative stress biomarkers, antioxidant defense parameters, protein oxidation indices, apoptotic activity (caspase-3), pro-inflammatory cytokines (IL-1, IL-6), and cell viability (MTT assay) were assessed. Under the applied conditions, all materials induced only limited global oxidative stress, with most alterations reflecting selective protein and glycoxidative modifications. Nevertheless, AH Plus, MTA Fillapex, and the calcium hydroxide-based Biopulp exhibited a less favorable redox profile and greater protein oxidation compared with calcium silicate-based sealers. AH Plus and EndoSeal MTA were associated with increased IL-6 release, whereas EndoSeal MTA moderately elevated IL-1 levels. BioRoot TM demonstrated the lowest cytokine expression, and TotalFill BC preserved high cell viability. Caspase-3 activity remained comparable across all experimental groups, indicating minimal induction of apoptosis.

Indexed as

apoptosisbioceramic sealersbiocompatibilitycytokine expressioncytotoxicityhuman periodontal ligament fibroblastsinflammatory responsein vitro studyoxidative stressredox balance

Identifiers

PMID41745567
PMCPMC12942283

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.