Evidence map›Paper›PMID 41746794›Full record

ArticleVascular biology (Bristol, England)2026

Endothelial RhoGEF Trio is involved in angiogenesis.

Jeffrey Kroon, Ilse Timmerman, Ilkka Paatero, Mark Hoogenboezem, Serge A van de Pavert, Ester M Weijers, Pieter Koolwijk, Heinz-Georg Belting, Jaap D van Buul

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vascular biology (Bristol, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jeffrey KroonDepartment of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.ORCID https://orcid.org/0000-0001-9983-6614
Ilse TimmermanDepartment of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Ilkka PaateroBiozentrum Universität Basel, Basel, Switzerland.
Mark HoogenboezemDepartment of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Serge A van de PavertDepartment of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.
Ester M WeijersDepartment of Physiology, Amsterdam UMC, Location VU University Medical Center, Amsterdam, The Netherlands.
Pieter KoolwijkDepartment of Physiology, Amsterdam UMC, Location VU University Medical Center, Amsterdam, The Netherlands.
Heinz-Georg BeltingBiozentrum Universität Basel, Basel, Switzerland.
Jaap D van BuulDepartment of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.ORCID https://orcid.org/0000-0003-0054-7949

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endothelial cells lining the vessel wall are instrumental in angiogenesis. They initiate this process and remodel their actin cytoskeleton to facilitate proliferation and migration from pre-existing vessels. However, the mechanisms that coordinate the remodeling of the endothelial actin cytoskeleton to promote angiogenesis are not fully understood. Here, we show that the RhoGEF Trio is involved in angiogenic sprouting in vitro and in vivo. Inhibition of Trio activity reduces sprout formation of intersomitic vessels in embryonic zebrafish. Moreover, a strong reduction in the length of developing sprouts is observed in a murine retinal explant assay. In addition, embryonic lethally Trio-/- mice show impaired disposition of the vasculature. Mechanistically, we show through rescue experiments that the N-terminal part of Trio, including the GEF1 domain, supported endothelial sprouting. Together, we conclude that Trio is involved in angiogenesis.

Indexed as

angiogenesisGEFRac1sproutingTrioVE-cadherin

Identifiers

PMID41746794
PMCPMC12978690

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.