Evidence map›Paper›PMID 41748865›Full record

ArticleScientific reports2026

Visualizing malignant progression: in situ CD109-based spatial immunofluorescence assay delineates papillary to anaplastic thyroid carcinoma transformation within the tumor microenvironment.

Tomoko Cohen, Keiji Suzuki, Katsuya Matsuda, Hirokazu Kurohama, Yuki Matsuoka, Mayu Ueda, Shinya Satoh, Hisakazu Shindo, Hiroyuki Yamashita, Atsushi Kawakami and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Tomoko CohenDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
Keiji SuzukiDepartment of Radiation Medical Sciences, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, 852-8523, Japan.
Katsuya MatsudaDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
Hirokazu KurohamaDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
Yuki MatsuokaDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
Mayu UedaDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan.
Shinya SatohYamashita Thyroid Hospital, Fukuoka, 812-0034, Japan.
Hisakazu ShindoYamashita Thyroid Hospital, Fukuoka, 812-0034, Japan.
Hiroyuki YamashitaYamashita Thyroid Hospital, Fukuoka, 812-0034, Japan.
Atsushi KawakamiDepartment of Endocrinology and Metabolism, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8501, Japan.
Masahiro NakashimaDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, 852-8523, Japan. moemoe@nagasaki-u.ac.jp.ORCID http://orcid.org/0000-0002-9036-8735

Funding

a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture 22K06982a Grant-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture 23K06465
6 · The paper itself

Abstract

Anaplastic thyroid carcinoma (ATC) is the rarest and most aggressive subtype of thyroid cancer, and considered to arise from differentiated thyroid carcinoma, however, the underlying molecular processes remain largely unknown. Using CD109 as a malignant marker, we analyzed an ATC case containing a papillary thyroid carcinoma (PTC) component. Our newly developed spatial immunofluorescence (SPI) assay, which enabled the differential expression of CD109 and CK8/18, a PTC marker, demonstrated gradual and completely opposed changes at the boundary between ATC and PTC components. Similar specific expression patterns were observed in E-cadherin, vimentin, PCNA, αSMA, Iba-1, collagen (COL) III/VI, TGFβ1-induced (TGFBI), active Yes-associated protein, periostin, and S100. The zonal and reciprocal transitions between PTC and ATC markers suggested that anaplastic transformation was not merely the result of clonal expansion, but rather it was executed by ATC-specific tumor microenvironment (ATC-TME) that recruited more Iba-1- and S100-positive macrophages, along with unique ATC-cancer-associated fibroblasts (CAFs), which deposited more COL III/VI and TGFBI. We proposed that expansion of ATC-CAFs caused extracellular matrix stiffening and compromised PTC cells, thereby inducing necroptosis and S100 release. This process simultaneously promoted the epithelial-mesenchymal transition in PTC cells and selected pre-existing PTC cells harboring additional gene mutations sufficient for anaplastic transformation.

Indexed as

Cell Transformation, NeoplasticThyroid Cancer, PapillaryThyroid Carcinoma, AnaplasticThyroid NeoplasmsTumor MicroenvironmentBiomarkers, TumorDisease ProgressionFluorescent Antibody TechniqueHumansBiomarkers, TumorAnaplastic thyroid cancerCD109Matrix stiffeningSpatial immunofluorescence assayTumor microenvironment

Identifiers

PMID41748865
PMCPMC12979697

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.