Evidence map›Paper›PMID 41755834›Full record

ArticleJACS Au2026

A Cryptic Pocket Allosterically Modulates Oligosaccharide Binding to DC-SIGN.

Jonathan Lefèbre, Maurice Besch, Marcelo Daniel Gamarra, Jan-Oliver Kapp-Joswig, Annika Balke, Stevan Aleksić, Henry Flatau, Gregor Suchy, Elena Georgieva, Patrick Scheerer and 3 more

Abstract read
In one paragraph

Article in JACS Au, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Synthetic Ligands of Myeloid C-Type Lectin Receptors.Chembiochem : a European journal of chemical biology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jonathan LefèbreDepartment of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.ORCID https://orcid.org/0000-0001-8505-2181
Maurice BeschDepartment of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.ORCID https://orcid.org/0000-0001-9242-0261
Marcelo Daniel GamarraDepartamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (FCEyN-UBA), Ciudad de Buenos Aires C1428EGA, Argentina.
Jan-Oliver Kapp-JoswigDepartment of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Arnimallee 22, Berlin 14195, Germany.ORCID https://orcid.org/0000-0003-1492-3757
Annika BalkeInstitute of Medical Physics and Biophysics, Group Structural Biology of Cellular Signaling, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin 10117, Germany.
Stevan AleksićDepartment of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Arnimallee 22, Berlin 14195, Germany.
Henry FlatauDepartment of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.
Gregor SuchyDepartment of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.
Elena GeorgievaDepartment of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Arnimallee 22, Berlin 14195, Germany.
Patrick ScheererInstitute of Medical Physics and Biophysics, Group Structural Biology of Cellular Signaling, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin 10117, Germany.ORCID https://orcid.org/0000-0001-5028-2075
Bettina G KellerDepartment of Biology, Chemistry, Pharmacy, Freie Universität Berlin, Arnimallee 22, Berlin 14195, Germany.ORCID https://orcid.org/0000-0002-7051-0888
Carlos Pablo ModenuttiDepartamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (FCEyN-UBA), Ciudad de Buenos Aires C1428EGA, Argentina.
Christoph RademacherDepartment of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.ORCID https://orcid.org/0000-0001-7082-7239

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DC-SIGN is a C-type lectin receptor expressed on antigen-presenting cells that is crucial for pathogen recognition and immune modulation. Here, we identify and characterize a previously unrecognized cryptic allosteric pocket in DC-SIGN using molecular dynamics simulations, NMR spectroscopy, cryogenic electron microscopy, and biochemical assays. Rotation of the gatekeeper residue M270 exposes the pocket whose occupancy modulates glycan binding. Mutations M270F and T314A mimic the occupied and unoccupied states of this pocket, respectively, shifting the conformational equilibrium of α-helix 2 and altering the oligosaccharide affinity via the extended carbohydrate binding site. While Ca

Indexed as

allosterycryptic pocketC-type lectinDC-SIGNNMR

Identifiers

PMID41755834
PMCPMC12933331

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.