Evidence map›Paper›PMID 41757349›Full record

ArticleFrontiers in cellular neuroscience2026

Liquid-liquid phase separation couples MKRN2-mediated ubiquitination of CSDE1 with neurodevelopmental disorders.

Zi Wang, Yaning Han, Peng Yang, Caiwei Jia, Chuanyin Li, Shilin Yuan, Pengfei Wei, Ronggui Hu

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In one paragraph

Article in Frontiers in cellular neuroscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Zi Wang *National Center for Mental Disorders, Shanghai Mental Health Center, Brain Health Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yaning Han *Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, Department of Pharmacology, SUSTech Homeostatic Medicine Institute, School of Medicine, Southern University of Science and Technology, Shenzhen, China.
Peng Yang *School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Caiwei JiaState Key Laboratory of Molecular Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Chuanyin LiDepartment of Colorectal Surgery and Oncology (Key Laboratory of Cancer and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Shilin YuanState Key Laboratory of Molecular Biology, Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Pengfei WeiState Key Laboratory of Digital Medicine, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
Ronggui HuNational Center for Mental Disorders, Shanghai Mental Health Center, Brain Health Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Makorin-2 (MKRN2) is an E3 ubiquitin ligase involved in multiple biological processes, yet its role in neurological disorders remains poorly understood. This study aims to elucidate how MKRN2 regulates the RNA-binding protein CSDE1-a molecule linked to autism-related genes-and to explore the functional implications of this interaction in neurodevelopment. Methods: Using mass-spectrometry screening, we identified CSDE1 as a direct substrate of MKRN2. Ubiquitination sites were validated through mutagenesis of conserved lysine residues. Liquid-liquid phase separation (LLPS) assays were performed in HEK293 and SH-SY5Y cells, and behavioral phenotypes were assessed in Results: MKRN2 mediates CSDE1 ubiquitination at four lysine residues (K81, K91, K208, K727). Deletion of Conclusion: Our study reveals an LLPS-coupled ubiquitination mechanism by which MKRN2 regulates CSDE1, providing a novel molecular pathway underlying neurodevelopmental disorders. These findings offer new insights for understanding and treating neurological diseases such as ASD.

Indexed as

autism spectrum disorderCsde1liquid–liquid phase separationMKRN2ubiquitination

Identifiers

PMID41757349
PMCPMC12932526

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.