Evidence map›Paper›PMID 41758230›Full record

ArticleAnalytical and bioanalytical chemistry2026

Effect of HDL disk and LDL dimer presence on lipoprotein particle number determination and subclassification.

Zsuzsanna Kuklenyik, Anna A Ivanova, Lauren E Drinkard, David M Schieltz, Jeffrey I Jones, Kevin Bierbaum, Christopher A Toth, Michael S Gardner, Bryan A Parks, Michael Andrews and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Zsuzsanna KuklenyikClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0001-9801-7705
Anna A IvanovaClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0002-6221-6240
Lauren E DrinkardClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
David M SchieltzClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0009-0006-8016-0961
Jeffrey I JonesClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
Kevin BierbaumClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
Christopher A TothClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
Michael S GardnerClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0003-0999-2010
Bryan A ParksClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0001-7026-873X
Michael AndrewsClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0003-1958-909X
Jennifer D KusovschiClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0002-7299-2454
Jack A SultanClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
Antony LehtikoskiClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.
Jon C ReesClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0003-0616-0219
Wanda I SantanaClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0000-0002-8602-6732
Victoria ConnorClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA.ORCID http://orcid.org/0009-0002-0887-9091
Eric C LeszczynskiUniversity of South Carolina, 921 Assembly Street, Columbia, SC, 29201, USA.ORCID http://orcid.org/0000-0002-1527-7374
Robert W McGarrahDuke Molecular Physiology Institute, Duke University School of Medicine, Duke University, Durham, NC, 27701, USA.
Mark A SarzynskiUniversity of South Carolina, 921 Assembly Street, Columbia, SC, 29201, USA.ORCID http://orcid.org/0000-0001-6705-0668
William E KrausDuke Molecular Physiology Institute, Duke University School of Medicine, Duke University, Durham, NC, 27701, USA.
John R BarrClinical Chemistry Branch, Division of Laboratory Sciences, Centers for Disease Control and Prevention, 4770 Buford Highway, Atlanta, GA, 30341, USA. jbb0@cdc.gov.ORCID http://orcid.org/0000-0001-6431-2175

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-density and low-density lipoproteins (HDL and LDL) are established analytical targets for diagnosis and risk stratification of numerous chronic diseases. This study investigates potential sources of bias in lipoprotein particle counting (HDL-P and LDL-P), focusing on the most atheroprotective small-HDL and most pro-atherogenic small-LDL. Plasma samples were fractionated using asymmetric-flow field-flow fractionation (AF4), coupled with hydrodynamic size measurement and comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of each fraction. Concentration-size profiles were deconvoluted into 10 HDL and 8 LDL Gaussian subspecies. Molecular volume ratios were used to evaluate proposed particle models, providing evidence for the presence of s-HDL disk and s-LDL dimers, as sources of bias in calculated HDL-P and LDL-P when spherical particle geometry is assumed. Matching apoA1/HDL-P and apoB/LDL-P to consensus values enabled correction of mass diameters (k*d

Indexed as

Lipoproteins, HDLLipoproteins, LDLChromatography, LiquidFractionation, Field FlowHumansLiquid Chromatography-Mass SpectrometryParticle SizeTandem Mass SpectrometryLipoproteins, HDLLipoproteins, LDLApolipoprotein compositionAsymmetric-flow field-flow fractionationHDLLDLLipid homeostasisLipoprotein particle numbersLipoprotein particlesLipoprotein particle size measurement

Identifiers

PMID41758230
PMCPMC13079496

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.