Evidence mapPaperPMID 41764265Full record

ArticleNature communications2026

Dynamic subcellular proteomics identifies regulators of adipocyte insulin action.

Olivia J Conway, Josie A Christopher, Lisa M Breckels, Hanqi Li, Dilip Menon, Meghna Birla, Bethan L Hawkins, Lu Liang, Satish Patel, Francoise Koumanov and 5 more

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Olivia J ConwayMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.
Josie A ChristopherCambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-7077-4894
Lisa M BreckelsCambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0001-8918-7171
Hanqi LiCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.
Dilip MenonMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.
Meghna BirlaMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.
Bethan L HawkinsMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0009-0003-0019-102X
Lu LiangMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.
Satish PatelMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-5345-8942
Francoise KoumanovCentre for Nutrition, Exercise and Metabolism, Department for Health, University of Bath, Bath, UK.ORCID http://orcid.org/0000-0002-9029-3390
David C GershlickCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-0602-210X
David B SavageMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK.
Michael P WeekesCambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0003-3196-5545
Kathryn S LilleyCambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0003-0594-6543
Daniel J FazakerleyMetabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Cambridge, UK. djf72@cam.ac.uk.ORCID http://orcid.org/0000-0001-8241-2903

Funding

EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020) project 823839RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/R505304/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/W005905/1RCUK | Medical Research Council (MRC) MR/S007091/1RCUK | Medical Research Council (MRC) MR/X000516/1RCUK | Medical Research Council (MRC) MR/X00970X/1RCUK | Medical Research Council (MRC) MR/Z504592/1Wellcome Trust 210481Wellcome Trust 219417Wellcome Trust (Wellcome) Wellcome Trust PhD studentship
6 · The paper itself

Abstract

Insulin acts on adipocytes to suppress lipolysis and increase glucose uptake to control whole-body glucose and lipid metabolism. Regulation of these processes by insulin signalling depends on changes in protein localisation. However, the extent of insulin-stimulated changes to the adipocyte spatial proteome, and the importance of these in the cellular insulin response, is unknown. Here, we use subcellular proteomics approaches to map acute insulin-stimulated protein relocalisation in adipocytes on a cell-wide scale. These data reveal extensive insulin-regulated protein redistribution, with hundreds of insulin-responsive proteins. These include the uncharacterised protein C3ORF18, which redistributes to the plasma membrane in response to insulin. Studies in C3ORF18-depleted adipocytes suggest this protein is required to maintain adipocyte insulin sensitivity. Overall, our data highlight the scale of protein relocalisation in the adipocyte insulin response, and provide an accessible resource to inform further studies into how changes in protein localisation contribute to cellular insulin responses.

Indexed as

AdipocytesInsulinProteomics3T3-L1 CellsAnimalsCell MembraneHumansMiceProtein TransportProteomeSignal TransductionInsulinProteome

Identifiers

PMID41764265
PMCPMC13066455

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.