Evidence map›Paper›PMID 41764534›Full record

ArticleJournal of translational medicine2026

Spatial phosphoproteomics reveals regional phosphorylation features in liver cirrhosis progression.

Mengyao Wu, Huihui Tao, Huaizhou Chen, Qiang Yan, Yaoshuang Zou, Lingling Zhou, Tiantian Xu, Xuejia Zheng, Chunmei Wen, Shumei Zhang and 3 more

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Article in Journal of translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

13 authors.

Mengyao Wu *Anhui University of Science and Technology Medical School, Huainan, China.
Huihui Tao *Anhui University of Science and Technology Medical School, Huainan, China. uniquethh@aust.edu.cn.
Huaizhou ChenThe Organ Transplantation Department of 924th Hospital of Joint Logistic Support Force of PLA, Guilin, China.
Qiang YanThe Organ Transplantation Department of 924th Hospital of Joint Logistic Support Force of PLA, Guilin, China.
Yaoshuang ZouThe Organ Transplantation Department of 924th Hospital of Joint Logistic Support Force of PLA, Guilin, China.
Lingling ZhouAnhui University of Science and Technology Medical School, Huainan, China.
Tiantian XuAnhui University of Science and Technology Medical School, Huainan, China.
Xuejia ZhengThe First Hospital of Anhui University of Science and Technology, Huainan, China.
Chunmei WenAnhui University of Science and Technology Medical School, Huainan, China.
Shumei ZhangAnhui University of Science and Technology Medical School, Huainan, China.
Ruijie YangAnhui University of Science and Technology Medical School, Huainan, China.
Xin WangAnhui University of Science and Technology Medical School, Huainan, China.
Yong DaiAnhui University of Science and Technology Medical School, Huainan, China. daiyong22@aust.edu.cn.ORCID 0000-0002-6840-9158

Funding

Anhui Provincial Clinical Medicine Research and Translational Special Project 202427b10020103Anhui Provincial Clinical Medicine Research and Translational Special Project 202427b10020132he Open Research Fund of the State Key Laboratory of Cellular Stress Biology, Xiamen University No. SKLCSB2024KF004
6 · The paper itself

Abstract

backgroundLiver cirrhosis (LC) is the end stage of chronic liver disease and is characterized by pseudolobule formation and extensive fibrosis. Protein phosphorylation, a key posttranslational modification, regulates cellular functions and disease progression. However, the dynamic changes in phosphorylation across different regions of cirrhotic liver tissue remain largely unexplored, thereby limiting mechanistic insights into LC pathogenesis.

methodsWe employed spatial phosphoproteomics combined with bioinformatics to analyze phosphorylation patterns in three LC tissue regions: the normal hepatocyte region (NH), the pseudolobular hepatocyte region (PH), and the fibrotic-inflammatory portal region (PII). The tissue samples were processed via laser capture microdissection (LCM), followed by phosphopeptide enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differential expression analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction (PPI) network construction, and kinase-substrate prediction were conducted to characterize spatial phosphorylation dynamics.

resultsKEGG analysis revealed significant enrichment of ribosome, actin cytoskeleton regulation, and focal adhesion pathways among the upregulated differentially expressed phosphoproteins (DEPPs) from the PH/NH and PII/PH comparisons, whereas spliceosome alterations were observed among upregulated DEPPs of PH/NH and downregulated DEPPs of PII/PH. This study has identified several candidate molecules awaiting further validation, including the phosphorylated proteins IQGAP1 and VIM, as well as the kinases ERK and GSK3α, which may be implicated in the progression of LC.

Indexed as

Disease ProgressionLiver CirrhosisPhosphoproteinsProteomicsChromatography, LiquidHumansLaser Capture MicrodissectionPhosphorylationProtein Interaction MapsTandem Mass SpectrometryPhosphoproteinsLaser capture microdissectionLiver cirrhosis progressionPhosphorylation changesSpatial phosphoproteomics

Identifiers

PMID41764534
PMCPMC13049880

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.