Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell.

RBK cell line, derived from primary bovine kidney cell, was established and introduced by Razi Vaccine and Serum Research Institute. RBK cell line has been transformed into continuous cell line through serial passaging of primary cell. Based on experiments conducted, the RBK cell line demonstrates high sensitivity to viruses. As cell line identification is crucial to confirm the validity of experimental results, and can ensure their credibility, accurate authentication helps prevent unreliable findings. Misidentification of the cell line or its contamination can lead to unreliable results as well as waste of resources. Forecasting the origin of a cell line, as it directly contributes to its value; the more detailed the origin, the more valuable the cell line is considered. Contamination is a serious concern in cell culture because it can significantly affect the physiology and viability of cell lines. Therefore, based on standard guidelines, it is necessary to implement various assays to ensure contaminant-free cultures and confirm the identity of cell lines. For accurate identification, verification and authentication of a cell line, various methods must be applied simultaneously. In this study, purification and identification of RBK cell line were investigated using molecular tests such as PCR, PCR-RFLP and real time PCR based on mitochondrial DNA. The present study provides the authentication of RBK cell line and demonstrates that the RBK is free of adventitious agents, including Bovine Leukemia Virus (BLV) and Bovine Viral Diarrhea (BVDV). Considering the RBK cell line's capability for unlimited proliferation, stability and sensitivity to viruses, the present study introduces this cell line as a reliable tool for viral research, which can contribute to numerous important scientific advances.