Evidence mapPaperPMID 41770518Full record

ArticleClinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico2026

ANKRD1 expression and functional mechanism in stomach adenocarcinoma.

Lu Nie, Yu Nie, Ruiyang Wang

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Article in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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3 authors.

Lu Nie *Department of Gastrointestinal Oncology, Shandong Cancer Hospital and Institute Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Yu Nie *Radiotherapy Department, Shandong Second Provincial General Hospital, Jinan, Shandong, China.
Ruiyang WangDepartment of Emergency, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No.1 Jingba Road, Shizhong District, Jinan, 250001, Shandong, China. wruiyang@126.com.ORCID http://orcid.org/0000-0002-6800-5698

Funding

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6 · The paper itself

Abstract

backgroundANKRD1 is implicated in various cancers, but its role in stomach adenocarcinoma (STAD) remains unclear.

methodsANKRD1 expression and its prognostic value in STAD were analyzed using TIMER, UALCAN, GEPIA, and Kaplan-Meier plotter. Immune cell infiltration was evaluated via CIBERSORT and Single-sample Gene Set Enrichment Analysis (ssGSEA). Somatic mutations were analyzed from TCGA data. Functional enrichment analysis (GO, KEGG, GSEA (Gene Set Enrichment Analysis)) was performed on ANKRD1-associated genes. Subsequently, in vitro experiments were conducted. ANKRD1 protein levels were examined in STAD cell lines by Western blot. Stable knockdown and overexpression models were created. Functional assays (CCK-8, Transwell, and wound healing) assessed proliferation, migration, and invasion. Western blot measured STAT3 pathway activity.

resultsANKRD1 was significantly overexpressed in STAD tissues, and high expression correlated with poorer overall, first progression, and post-progression survival. ANKRD1 expression positively correlated with M0 macrophage and activated mast cell infiltration and negatively with resting memory CD4 + T cells and naive B cells. Although ANKRD1 itself was not mutated, associated genes were enriched in pathways like Wnt signaling. In vitro, ANKRD1 knockdown inhibited cell proliferation, migration, and invasion, while its overexpression promoted these effects. ANKRD1 was found to modulate STAT3 phosphorylation.

conclusionsANKRD1 is overexpressed in STAD and predicts poor prognosis. It promotes tumor cell proliferation, migration, and invasion, likely through activating the STAT3 signaling pathway, and correlates with an altered immune microenvironment.

Indexed as

AdenocarcinomaMuscle ProteinsNuclear ProteinsRepressor ProteinsStomach NeoplasmsCell Line, TumorCell MovementCell ProliferationFemaleGene Expression Regulation, NeoplasticHumansMast CellsNeoplasm InvasivenessPrognosisSTAT3 Transcription FactorANKRD1 protein, humanMuscle ProteinsNuclear ProteinsRepressor ProteinsSTAT3 protein, humanSTAT3 Transcription FactorANKRD1 (ankyrin repeat domain 1)Cell invasionSTAT3 signalingStomach adenocarcinomaTumor immune microenvironmentWestern blot

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.