Evidence map›Paper›PMID 41771865›Full record

ArticleNature communications2026

Generation of multitissue cell-cultivated meat via multidirectional differentiation of stable porcine epiblast stem cells.

Yixuan Yao, Gaoxiang Zhu, Minglei Zhi, Runbo Li, Lun Qin, Yu Zhang, Yachun Chen, Xinze Chen, Tong Wang, Linzi Li and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Yixuan Yao *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Gaoxiang Zhu *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Minglei Zhi *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.ORCID http://orcid.org/0000-0002-0609-869X
Runbo Li *State Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Lun QinAnimal Science and Technology College, Beijing University of Agriculture, Beijing, People's Republic of China.
Yu ZhangSchool of Food and Health, Beijing Technology and Business University, Beijing, China.
Yachun ChenSchool of Food and Health, Beijing Technology and Business University, Beijing, China.
Xinze ChenState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Tong WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Linzi LiCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shanxi, China.
Yingjie WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Shunxin WangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
He ZhangState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China.
Xianchao FengCollege of Food Science and Engineering, Northwest A&F University, Yangling, Shanxi, China.ORCID http://orcid.org/0000-0002-8602-207X
Aijin MaSchool of Food and Health, Beijing Technology and Business University, Beijing, China. maaj@btbu.edu.cn.
Suying CaoAnimal Science and Technology College, Beijing University of Agriculture, Beijing, People's Republic of China. 20137602@bua.edu.cn.
Jianyong HanState Key Laboratory of Animal Biotech Breeding, Frontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, People's Republic of China. hanjy@cau.edu.cn.ORCID http://orcid.org/0000-0002-9549-3279

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-cultivated meat has emerged as an alternative approach for sustainable protein production, but replicating the texture and complex composition of conventional meat remains a critical challenge. Here, we developed an efficient approach to produce multitissue engineered meat by leveraging a serum- and animal-component-free differentiation system to direct porcine pregastrulation epiblast stem cells toward muscle, adipose, and endothelium with synergistic functions. These three types of cell progenitors demonstrated autonomous intercellular recognition and coculture compatibility in a scaffold-free 3D suspension system, enabling the spontaneous formation of tissue-mimetic spheroids with enhanced cellular multiplication efficiency. The products of multitissue cultivated meat recapitulated the textural properties of conventional pork and allowed for nutritional modulation. This platform overcomes key limitations in currently employed cultured meat technologies by integrating scalable 3D suspension culture with serum-free, species-specific stem cell multidirectional differentiation, providing an avenue for the development of multitissue cell-cultivated meat.

Indexed as

Cell DifferentiationGerm LayersStem CellsTissue EngineeringAdipose TissueAnimalsCell Culture TechniquesCells, CulturedCoculture TechniquesIn Vitro MeatSwine

Identifiers

PMID41771865
PMCPMC13066626

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.