Evidence map›Paper›PMID 41775853›Full record

ArticleScientific reports2026

Müller glia derived EVs promote neurite recovery of an enriched population of retinal ganglion like cells derived from hESC retinal organoids after damage.

Karen Eastlake, William D B Lamb, Dhani Tracey-White, Keiichi Shibagaki, Peng T Khaw, Hari Jayaram, G Astrid Limb

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Karen EastlakeNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK. k.eastlake@ucl.ac.uk.
William D B LambNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK.
Dhani Tracey-WhiteNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK.
Keiichi ShibagakiDiscovery Research, Ophthalmology Innovation Center, Pipeline Creation, Santen Pharmaceutical Co. Ltd., Osaka, Japan.
Peng T KhawNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK.
Hari JayaramNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK.
G Astrid LimbNational Institute for Health and Care Research (NIHR) Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, 11-43 Bath Street, EC1V 9EL, London, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane-bound extracellular vesicles (EVs) released by Müller glia contain microRNA (miRNA) and proteins with the potential to be beneficial in providing neuroprotection in retinal degenerative conditions. The aim of this study was to examine the neuroprotective effect of Müller glia derived EVs in human ESC-derived neuronal cells containing RGCs. Cells were isolated from 40–50-day-old retinal organoids and cytotoxicity was induced by addition of NMDA (1mM) for 24 h, followed by treatment with a population of Müller cell derived EVs for a further 24 h. Isolated RGC-enriched cultures expressed characteristic RGC markers such as βIII tubulin, Brn3b, RBPMS, γ-synuclein, Thy1 and NMDAR1. Exposure of this cell population to NMDA lead to a significant reduction in the average neurite length which then recovered with exposure to Müller cell-derived EVs. Investigation of kinase activity revealed increased apoptotic signalling via activation of P38 and p53 after NMDA addition to RGC-like cultures and pro-survival signalling by activation of RSK1/2/3 after addition of EV to the NMDA-damaged cells, suggesting EVs derived from Müller glia support the survival of the RGC-enriched cultures. It is hoped that these observations aid future investigations to assess new EV-related therapies to treat neuronal injury occurring in retinal degenerative conditions such as glaucoma.

Indexed as

Ependymoglial CellsNeuritesOrganoidsRetinal Ganglion CellsApoptosisCells, CulturedCell SurvivalHumansN-MethylaspartateRetinaN-MethylaspartateDegenerationMuller gliaNeuroprotectionRetinaRetinal ganglion cellsRetinal organoidsStem cells

Identifiers

PMID41775853
PMCPMC13065809

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.