Evidence map›Paper›PMID 41776121›Full record

ArticleDiscover oncology2026

HPV status determines prognostic gene expression methylation and immune infiltration in head and neck squamous cell carcinoma.

Yifan Yang, Lingwa Wang, Ru Wang, Haiyang Li, Siyu Zhu, Jugao Fang, Ling Feng

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PubMed Full text at PMC Publisher
In one paragraph

Article in Discover oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yifan YangDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China.ORCID http://orcid.org/0000-0001-5582-5147
Lingwa WangDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China.ORCID http://orcid.org/0000-0001-6771-5439
Ru WangDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China.ORCID http://orcid.org/0000-0003-4402-4533
Haiyang LiDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China.ORCID http://orcid.org/0000-0002-2773-4085
Siyu ZhuDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China.ORCID http://orcid.org/0009-0002-3295-2236
Jugao FangDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, No.1 Dongjiaominxiang Street, Beijing, 100730, China. fangjugao2@ccmu.edu.cn.ORCID https://orcid.org/0000-0002-0222-1195
Ling FengDepartment of Otorhinolaryngology, Head and Neck Surgery, Beijing Shijitan Hospital, Capital Medical University, No.10 Tieyi Road, Beijing, 100038, China. fengling@ccmu.edu.cn.ORCID https://orcid.org/0009-0001-6533-4795

Funding

Beijing Hospitals Authority Youth Programme QML20230206Beijing Natural Science Foundation 7244308
6 · The paper itself

Abstract

purposeHead and neck squamous cell carcinoma (HNSCC) continues to be a deadly cancer with heterogeneous molecular characteristics and poor survival outcomes, particularly in HPV- patients. This study aimed to identify key overexpressed genes that drive HNSCC progression, evaluate their prognostic value, and explore associations with HPV status, promoter methylation, and changes in the immune microenvironment.

methodsUsing bioinformatics tools (UALCAN, HPA, TISIDB), we analyzed TCGA-HNSC data to evaluate gene expression, survival correlations, HPV subgroup differences, promoter methylation, and immune infiltration patterns. Statistical significance was defined as p < 0.05.

resultsAmong the top 50 overexpressed genes in HNSCC, eight (LAMC2, CDKN2A, MFAP2, CTHRC1, CXCL13, FST, SPP1, PLAU) exhibited significant survival associations (p < 0.05). HPV- tumors demonstrated marked upregulation of LAMC2, CTHRC1, FST, SPP1, and PLAU, alongside downregulation of CDKN2A and CXCL13. Promoter hypomethylation in tumor tissues correlated with overexpression of LAMC2, CTHRC1, CXCL13, FST, SPP1, and PLAU, whereas CDKN2A showed hypermethylation. Immune infiltration analysis revealed strong correlations between these genes and immunosuppressive Tregs or cytotoxic T-cell depletion.

conclusionThis study identifies eight prognostic biomarkers in HNSCC linked to HPV-driven heterogeneity. These genes could be potential targets for therapy in combination with immunotherapy and epigenetic regulators, helping to overcome tumor resistance in HNSCC with different HPV status.

Indexed as

DatabaseDNA methylationHead and neck squamous cell carcinomaHPVTumor immune microenvironment

Identifiers

PMID41776121
PMCPMC13065962

What Socratic holds

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.