Evidence map›Paper›PMID 41777401›Full record

ArticleParasite epidemiology and control2026

Diagnostic challenges in malaria detection: A comparative diagnostic performance of HRP2-based rapid diagnostic tests, microscopy, and PCR at Bichena primary hospital, Northwest Ethiopia.

Awoke Minwuyelet, Delenasaw Yewhalaw, Getnet Atenafu

Abstract read
In one paragraph

Article in Parasite epidemiology and control, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Awoke MinwuyeletDepartment of Biology, College of Natural and Computational Science, Debre Markos University, Debre Markos, Amhara, Ethiopia.
Delenasaw YewhalawTropical and Infectious Diseases Research Center, Jimma University, Jimma, Ethiopia.
Getnet AtenafuDepartment of Biology, College of Natural and Computational Science, Debre Markos University, Debre Markos, Amhara, Ethiopia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Accurate malaria diagnosis is crucial for effective case management, strong surveillance, and progress toward elimination. However, in highland regions, diagnostic tools are underutilized or yield suboptimal performance. While hematological alterations are frequently observed in malaria, their role remains largely supportive rather than diagnostic. This study aimed to evaluate diagnostic challenges by comparing the performance of HRP2-based rapid diagnostic tests, microscopy, and PCR at Bichena Primary Hospital, Northwest Ethiopia, with hematological profiles examined as supportive indicators to help contextualize diagnostic performance. Materials and methods: A facility-based cross-sectional study was conducted between 31/12/2024 to 28/02/2025, with 274 participants enrolled through consecutive sampling. Socio-demographic data were collected using semi-structured questionnaires. The diagnostic evaluation used nested polymerase chain reaction (PCR) (from dried blood spots), microscopy (capillary and venous blood), histidine rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs), and hematological profiling. Data analysis was carried out with Statistical Package for the Social Sciences (SPSS) version 25.0, assessing diagnostic accuracy through sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV), while inter-test agreement was measured using Cohen's Kappa coefficient. Results were summarized in text, figures, and tables. Results: Higher prevalence of Conclusions: Both HRP2-antigen based RDTs and microscopy demonstrated lower sensitivity compared to PCR. RDTs showed the lowest diagnostic potential for

Indexed as

Diagnostic challengeMalariaMicroscopyPCRP.FalciparumP.VivaxRDT

Identifiers

PMID41777401
PMCPMC12950475

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.