Evidence map›Paper›PMID 41788196›Full record

ArticleFrontiers in cellular neuroscience2026

ACSA2 is not astrocyte-specific: implications for cell sorting strategies in the rodent brain.

Emerson Daniele, Gabriel Khelifi, Daniel Beretta, Boyan K Tsankov, K W Annie Bang, Chiara Beretta, Dana J Philpott, Samer M Hussein, Maryam Faiz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular neuroscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Emerson DanieleInstitute of Medical Science, University of Toronto, Toronto, ON, Canada.
Gabriel KhelifiDepartment of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Université Laval, Québec City, QC, Canada.
Daniel BerettaDepartment of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Boyan K TsankovDepartment of Immunology, University of Toronto, Toronto, ON, Canada.
K W Annie BangNetwork Biology Collaborative Centre Flow Cytometry Facility, Sinai Health, Lunenfeld-Tanenbaum Research Institute, Toronto, ON, Canada.
Chiara BerettaDepartment of Cell Biology and Anatomy, Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
Dana J PhilpottInstitute of Medical Science, University of Toronto, Toronto, ON, Canada.
Samer M HusseinDepartment of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Université Laval, Québec City, QC, Canada.
Maryam FaizInstitute of Medical Science, University of Toronto, Toronto, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Astrocyte-specific cell surface antigen-2 (ACSA2) has been established as the gold-standard marker for isolating astrocytes via magnetic-activated cell sorting (MACS) or fluorescence-activated cell sorting (FACS) for downstream transcriptomic studies. In a prior study of the astrocyte response to cortical stroke, we used ACSA2-based cell sorting prior to single cell RNA sequencing (scRNAseq). We found a substantial population of ACSA2 Methods: An intravenous antibody labeling strategy coupled with flow cytometry was used to determine whether contamination originated from circulating immune cells or microglia. Results: Contaminating cells were identified as CNS-resident microglia that express CD45, CD11b, and ACSA2. Discussion: These findings caution against the usage of ACSA2 for astrocyte purification without exclusion markers to achieve high-purity astrocyte populations for downstream multi-omics analyses.

Indexed as

ACSA2astrocytebrain cell isolationFACSMACSscRNA-seq

Identifiers

PMID41788196
PMCPMC12956691

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.