Evidence map›Paper›PMID 41794696›Full record

ArticleOrphanet journal of rare diseases2026

Characterisation of the wound microbiome and antimicrobial resistance profiles in clinical isolates from epidermolysis bullosa patients.

Anteneh Amsalu, Hanif Haidari, Bianca Mirco, Victoria Rudolph-Stringer, Sophie Walter, Anna Antipov, Dédée F Murrell, Zlatko Kopecki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Orphanet journal of rare diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anteneh AmsaluFuture Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia.
Hanif HaidariCollege of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Australia.
Bianca MircoDepartment of Dermatology, St. George Hospital, Sydney, Australia.
Victoria Rudolph-StringerDepartment of Dermatology, St. George Hospital, Sydney, Australia.
Sophie WalterDepartment of Dermatology, St. George Hospital, Sydney, Australia.
Anna AntipovFuture Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia.
Dédée F MurrellDepartment of Dermatology, St. George Hospital, Sydney, Australia.
Zlatko KopeckiFuture Industries Institute, University of South Australia, Mawson Lakes, SA, 5095, Australia. zlatko.kopecki@unisa.edu.au.ORCID http://orcid.org/0000-0002-8072-4222

Funding

Channel 7 Children's Research Foundation Channel 7 Children's Research FoundationDEBRA Australia DEBRA AustraliaEpidermolysis Bullosa Medical Research Foundation Epidermolysis Bullosa Medical Research Foundation
6 · The paper itself

Abstract

introductionEpidermolysis bullosa (EB) encompasses a rare, inherited group of skin disorders characterised by skin fragility, leading to painful, chronic wounds. While recent international studies have reported reduced microbial diversity and altered bacterial composition in EB blistered skins, no microbiome or antimicrobial resistance data is available for Australian EB patients. The objectives of this study were to characterise the wound microbiome of Australian EB patients and identify bacterial pathogens and their associated antimicrobial resistance profiles.

methodsA total of 15 chronic wound swabs were collected from 10 EB patients. Bacterial identification was performed using standard culture methods and MALDI-TOF; for a selected resistant subset, whole-genome sequencing (WGS) was conducted on two strains, and full-length 16S rRNA gene sequencing was performed on 10 swab samples. Antimicrobial susceptibility profiles were determined using disk diffusion assays.

resultPacBio Full-length 16S rRNA sequence analysis revealed a high relative abundance of the genera Staphylococcus and Streptococcus, with Staphylococcus aureus being the most frequently detected species. Among the 27 cultured bacterial isolates, 81.5% exhibited resistance to at least one antibiotic, and 22.2% were classified as multidrug resistance (MDR). WGS of two selected resistant strains harboured blaZ, mecA, ermC and mupA genes conferring resistance to penicillins, cefoxitin, clindamycin and high-level mupirocin, respectively.

conclusionsThis is the first study to profile the wound microbiome and antimicrobial resistance in Australian EB patients. The findings provide the descriptive microbial profiles and resistance patterns, with implications for clinical management of patients in a specific healthcare setting.

Indexed as

Epidermolysis BullosaMicrobiotaAdultAnti-Bacterial AgentsFemaleHumansMaleMicrobial Sensitivity TestsRNA, Ribosomal, 16SSkin MicrobiomeWhole Genome SequencingAnti-Bacterial AgentsRNA, Ribosomal, 16S16S rRNA sequencingAntimicrobial resistanceBlistered skinChronic woundEpidermolysis bullosaStaphylococcus aureusWound microbiome

Identifiers

PMID41794696
PMCPMC13081397

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.