Evidence map›Paper›PMID 41796379›Full record

ArticleStem cell research & therapy2026

SLIT3/ROBO1 axis contributes to angiogenic-osteogenic coupling in endothelial progenitor cells and peripheral blood mesenchymal stem cells.

Qiong Rong, Ling Ma, Mengting Wang, Qian Liu, Yali Zhang, Zhi Yuan, Xiaobing Tan

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Article in Stem cell research & therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Qiong Rong *Department of Stomatology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Rd, Kunming, 650032, Yunnan, China.
Ling Ma *Department of Clinical Pharmacy, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Mengting WangDepartment of Stomatology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Rd, Kunming, 650032, Yunnan, China.
Qian LiuKunming Health Vocational College, Kunming, China.
Yali ZhangThe First People's Hospital of Qujing, Qujing, China.
Zhi YuanDepartment of Stomatology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Rd, Kunming, 650032, Yunnan, China. 735632387@qq.com.
Xiaobing TanDepartment of Stomatology, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, 157 Jinbi Rd, Kunming, 650032, Yunnan, China. txb942005@163.com.ORCID http://orcid.org/0000-0002-2904-0765

Funding

Joint Fund for Applied Basic Research of Yunnan Provincial Science and Technology Department-Kunming Medical University 202201AY070001-231National Natural Science Foundation of China 82001007National Natural Science Foundation of China 82160178
6 · The paper itself

Abstract

backgroundEarly vascularization is one of the limitations of periodontal tissue engineering (PTE) based on mesenchymal stem cells (MSCs). Directed differentiation of endothelial progenitor cells (EPCs) into endothelial cells facilitates the osteogenic effect of MSCs. Therefore, this study constructed EPCs/peripheral blood derived-MSCs (EPCs/PBMSCs) sheets and evaluated their repair value and potential molecular mechanisms in bone regeneration.

methodsDifferent ratios of EPCs and PBMSCs were co-cultured to prepare EPCs/PBMSCs sheets and the osteogenic differentiation was assessed. Exploring the bone regeneration properties of EPCs/PBMSC sheets in an animal model of alveolar bone defects. The effect of the SLIT3/ROBO1 axis on angiogenic-osteogenic coupling of EPCs/PBMSCs sheets was explored using exogenous modulation by shRNA lentivirus and neutralizing antibody.

resultsEPCs/PBMSCs sheets could form angiogenic-osteogenic coupling, and different ratios of EPCs/PBMSCs sheets had higher angiogenic and osteogenic differentiation properties than EPCs or PBMSCs alone, especially the ratio 4:6. Moreover, EPCs/PBMSCs sheets accelerated bone regeneration in the alveolar bone defect model and the treatment was superior to PBMSCs alone. The expression patterns of SLIT3 and ROBO1 were consistent with the angiogenic-osteogenic coupling of EPCs/PBMSCs sheets. Knockdown of SLIT3 in PBMSCs and/or neutralization of ROBO1 protein in EPCs effectively suppressed calcified nodule formation and markers expression of osteogenic differentiation and angiogenesis (ALP, RUNX2, OCN, Osx, EMCN, and CD31) in EPCs/PBMSCs sheets, and hindered its therapeutic effect in the alveolar bone defect model.

conclusionEPCs/PBMSCs sheets ameliorate the limitations of early vascularization in PTE and the SLIT3/ROBO1 axis mediates the angiogenic-osteogenic coupling of EPCs/PBMSCs sheets, thereby augmenting their osteogenic effects.

Indexed as

AngiogenesisEndothelial Progenitor CellsMembrane ProteinsMesenchymal Stem CellsNeovascularization, PhysiologicNerve Tissue ProteinsOsteogenesisReceptors, ImmunologicAnimalsBone RegenerationCell DifferentiationCells, CulturedCoculture TechniquesHumansMaleRoundabout ProteinsMembrane ProteinsNerve Tissue ProteinsReceptors, ImmunologicRoundabout ProteinsSlit3 protein, mouseAngiopoiesisEndothelial progenitor cellMesenchymal stem cellOsteogenic differentiationTissue engineering

Identifiers

PMID41796379
PMCPMC13081268

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.