Evidence map›Paper›PMID 41801403›Full record

ArticlePediatric surgery international2026

Mesenchymal stromal cell therapy restores intestinal integrity and attentuates inflammation in a preterm piglet model of necrotizing enterocolitis.

Jasmine Lee, Sharon Joseph, Krishna Manohar, Fikir Mesfin, Chelsea Hunter, John Brokaw, W Chris Shelley, Jianyun Liu, Robyn McCain, Christa J Crain and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pediatric surgery international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Jasmine LeeDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Sharon JosephDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Krishna ManoharDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Fikir MesfinDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Chelsea HunterDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
John BrokawDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
W Chris ShelleyDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Jianyun LiuDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA.
Robyn McCainDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Christa J CrainDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Timothy LescunDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA.
Troy A MarkelDepartment of Surgery, Section of Pediatric Surgery, Indiana University School of Medicine, 705 Riley Hospital Dr., RI 2500, Indianapolis, IN, 46202, USA. tmarkel@iu.edu.

Funding

Sulfur Based Stem Cell Therapeutics in Necrotizing EnterocolitisR01DK133418 · NIDDK · INDIANA UNIVERSITY INDIANAPOLIS · PI TROY A MARKEL · 2023 to 2026
$1.9M
Chan Zuckerberg Initiative 2022-316749National Institute of Child Health and Human Development R01HD105301NIDDK NIH HHS R01DK133418Riley Children's Foundation NA
6 · The paper itself

Abstract

purposeNecrotizing enterocolitis (NEC) is a life-threatening gastrointestinal disease of prematurity characterized by inflammation, necrosis, and high morbidity. Current therapies are limited, necessitating the development of novel treatments. Mesenchymal stromal cells (MSCs) have shown promise in murine NEC models. Given the anatomical and physiological similarities between premature piglets and human infants, we employed a preterm piglet model to evaluate MSC efficacy. We hypothesized that intraperitoneal MSC administration would reduce intestinal injury in NEC.

methodsPreterm piglets were delivered via cesarean section. NEC was induced on day 3 through hypertonic enteral feeding. MSCs were administered intraperitoneally at low, medium, or high doses. Piglets were monitored and euthanized based on clinical criteria. Clinical scores, weight change, gross and histologic intestinal injuries were assessed. Cytokine levels in serum and ileum were quantified via ELISA, and intestinal tissue was analyzed by RNA sequencing. Statistical significance was set at p < 0.05.

resultsMedium-dose MSCs significantly improved clinical scores and reduced both gross and histologic intestinal injury (p < 0.05). A corresponding decrease in pro-inflammatory cytokines was observed.

conclusionThis is the first study to demonstrate therapeutic benefit of MSCs in a preterm piglet NEC model, supporting their potential use in translational NEC therapies.

Indexed as

Enterocolitis, NecrotizingMesenchymal Stem Cell TransplantationAnimalsAnimals, NewbornCytokinesDisease Models, AnimalFemaleInflammationSwineCytokinesInflammationMesenchymal stromal cellsNecrotizing enterocolitisPreterm pigletStem cell therapy

Identifiers

PMID41801403
PMCPMC12971755

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.