Evidence map›Paper›PMID 41808989›Full record

ArticlebioRxiv : the preprint server for biology2026

Intravital single-molecule imaging reveals cytoskeletal turnover as a driver of membrane remodeling in live animals.

Marco Heydecker, Desu Chen, Andrius Masedunskas, Melissa Mikolaj, Kedar Narayan, Jiji Chen, Harshad Vishwasrao, Tobias Meckel, Edna Hardeman, Peter Gunning and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marco HeydeckerLaboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0006-8409-7058
Desu ChenLaboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0003-4255-0361
Andrius MasedunskasSchool of Biomedical Sciences, University of New South Wales Sydney, Sydney, NSW, Australia.ORCID 0000-0002-4533-5467
Melissa MikolajCenter for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA and Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.ORCID 0000-0002-5452-1683
Kedar NarayanCenter for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA and Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
Jiji ChenAdvanced Imaging and Microscopy Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Harshad VishwasraoAdvanced Imaging and Microscopy Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0004-3188-1012
Tobias MeckelDepartment of Chemistry, Macromolecular Chemistry & Paper Chemistry, Technical University Darmstadt, Alarich-Weiss-Straße 8, 64287, Darmstadt, Germany.ORCID 0000-0003-0759-2072
Edna HardemanSchool of Biomedical Sciences, University of New South Wales Sydney, Sydney, NSW, Australia.ORCID 0000-0003-1649-7712
Peter GunningSchool of Biomedical Sciences, University of New South Wales Sydney, Sydney, NSW, Australia.ORCID 0000-0003-0833-3128
Roberto WeigertLaboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0003-0740-4465

Funding

WORK ORDER 126643 B539 EXPAND IC SUITE75N91019D00024 · NIAID · LEIDOS BIOMEDICAL RESEARCH, INC. · PI BRISCOE, LYNN · 2019 to 2025
$3932.6M
Molecular mechanisms of membrane remodelingZIABC011682 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI WEIGERT, ROBERTO · 2016 to 2025
$17.5M
Intramural NIH HHS ZIA BC011682NIH HHS 75N91019D00024
6 · The paper itself

Abstract

Understanding how cells regulate plasma membrane architecture inside intact living organs in a live animal has been limited by the inability to directly measure molecular dynamics in vivo. Here we introduce intravital single-molecule microscopy (iSiMM), an imaging approach that enables tracking of individual, endogenously expressed cytoskeletal components at the plasma membrane in live mice. Applying iSiMM to murine acinar secretory cells, we identify discrete basolateral membrane domains built on deeply folded membrane infolds that function as a pre-existing membrane reservoir. Single-molecule measurements reveal continuous, regulated molecular turnover within these domains. Physiological stimulation accelerates cytoskeletal exchange promoting rapid membrane unfolding and cell expansion. Together, these findings establish iSiMM as a general strategy for probing molecular kinetics underlying dynamic cellular behaviors in intact organs.

Identifiers

PMID41808989
PMCPMC12970338

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.