Evidence map›Paper›PMID 41811203›Full record

ArticleJournal of extracellular vesicles2026

Extracellular Vesicles From Mesenchymal Stromal Cells Drive Muscle and Neuronal Regeneration Through TNFα Modulation.

Agner Henrique Dorigo Hochuli, Stefania D'Agostino, Lucia Rossi, Beatrice Auletta, Leonardo Nogara, Giovanni Tafuro, Giuseppe Germano, Alice Zaramella, Paola Bisaccia, Francesca Cecchinato and 11 more

Abstract read
In one paragraph

Article in Journal of extracellular vesicles, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Agner Henrique Dorigo HochuliStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0003-2900-7655
Stefania D'AgostinoStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0001-6938-6851
Lucia RossiStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0009-0000-1703-1354
Beatrice AulettaDepartment of Industrial Engineering, University of Padova, Padova, Italy.ORCID https://orcid.org/0009-0003-2237-6690
Leonardo NogaraBiomedical Sciences Department, University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0002-7009-8707
Giovanni TafuroDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Giuseppe GermanoInstitute of Paediatric Research Città della Speranza, Padova, Italy.
Alice ZaramellaStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0002-5327-0325
Paola BisacciaStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0002-4533-0770
Francesca CecchinatoNeuromuscular engineering lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.
Carlo BizDepartment of Surgery, Oncology and Gastroenterology DiSCOG, Orthopaedic Clinic, University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0001-8517-0057
Gabrielis KundrotasEXO Biologics SA, Liège, Belgium.ORCID https://orcid.org/0000-0002-1222-6467
Pietro RuggieriDepartment of Surgery, Oncology and Gastroenterology DiSCOG, Orthopaedic Clinic, University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0001-9617-9882
Alessandra SemenzatoDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0003-2737-9869
Maurizio MuracaInstitute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0003-1218-097X
Filippo RomanatoDepartment of Physics "Galileo Galilei", University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0003-0243-1464
Piergiorgio GambaStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0003-2068-342X
Marcin JurgaEXO Biologics SA, Liège, Belgium.
Bert BlaauwBiomedical Sciences Department, University of Padova, Padova, Italy.ORCID https://orcid.org/0000-0002-4167-5106
Anna UrciuoloInstitute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0001-7571-9747
Michela PozzobonStem Cells and Regenerative Medicine Lab, Institute of Paediatric Research Città della Speranza, Padova, Italy.ORCID https://orcid.org/0000-0001-9213-402X

Funding

European Union's Horizon 2020 research and innovation program 797424Institute of Paediatric Research Città della Speranza, Padova, Italy 21/05Institute of Paediatric Research Città della Speranza, Padova, Italy 21/07Wallonie Recherche SPW 8357Wallonie Recherche SPW 8358
6 · The paper itself

Abstract

Muscle defects caused by accidents, tumour resection and congenital malformations affect paediatric and adult patients. In this context, the neural-muscle regeneration potential of mesenchymal stromal/stem cells derived from extracellular vesicles (EV) has been demonstrated by our group and others, but the mechanism by which EVs act remains unknown. This work aimed to investigate the neural-muscle regeneration mechanism shown by EVs in vivo using three-dimensional (3D) multicellular in vitro models. We used (1) human muscle decellularised tissue (ECM) engineered with human muscle precursor cells (hMPC) together with macrophages THP-1 (M0) and (2) organotypic spinal cord from rat foetuses. We also studied neuroinflammation in 2D with primary microglia cells stimulated with lipopolysaccharide (LPS). Samples treated with good manufacturing practices (GMP)-grade EVs were assessed, combining functional analyses, protein and gene expression. In the functional muscle model, EVs protect the cells from death after damage, decreasing cCAS3 and stimulating cell proliferation. The protein array and gene results highlighted that EVs act through the downregulation of the TNFα factor. In parallel, in both neuroinflammation-induced microglia and organotypic spinal cord-damaged models, EVs regulated the neuroinflammation by inhibiting TNFα and promoting neural axon sprouting. In summary, EVs guard great potential for tissue regeneration by TNFα modulation, promoting muscle-neural regeneration.

Indexed as

Extracellular VesiclesMesenchymal Stem CellsMuscle, SkeletalNerve RegenerationRegenerationTumor Necrosis Factor-alphaAnimalsCell ProliferationHumansMicrogliaRatsTumor Necrosis Factor-alpha3D modelbiological nanoparticlesfunctional muscle modelinflammationmicroRNAregenerative medicinetissue engineering

Identifiers

PMID41811203
PMCPMC12978157

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.