Evidence map›Paper›PMID 41811524›Full record

ReviewApoptosis : an international journal on programmed cell death2026

Looking at the fraction with Annexin V⁺ and propidium iodide⁺: insights into cell death types from preclinical studies in solid and haematological cancers.

Siti Nazihahasma Hassan, Farizan Ahmad

Abstract readReview
In one paragraph

Review in Apoptosis : an international journal on programmed cell death, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Therapeutic Potential ofInternational journal of molecular sciences · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Siti Nazihahasma HassanDepartment of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.ORCID 0000-0001-5263-1654
Farizan AhmadDepartment of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia. farizan@usm.my.ORCID 0000-0001-6413-7530

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescein isothiocyanate-conjugated Annexin V in combination with propidium iodide (PI) labelling is a widely used flow cytometric assay for quantifying apoptotic and necrotic cells in anticancer studies. However, increasing evidence suggests that double-positive cells, or the Annexin V⁺/PI⁺ fraction, may represent not only late apoptosis but also different modalities of regulated cell death, including necroptosis, pyroptosis, ferroptosis, and cuproptosis. By collating findings from preclinical studies across different cancer cells, this review highlights the need for consensus in interpreting Annexin V⁺/PI⁺ populations. In the absence of molecular and/or microscopy data, this fraction is more appropriately classified as undergoing 'late-stage cell death'. In short, establishing standardised interpretive criteria is crucial to enhance understanding, facilitate cross-study comparability, and improve the translational relevance of anticancer research.

Indexed as

Annexin A5ApoptosisHematologic NeoplasmsNeoplasmsPropidiumAnimalsCell DeathCuproptosisFerroptosisFlow CytometryHumansNecroptosisAnnexin A5PropidiumAnnexin vAnticancerApoptosisCancer cell deathPropidium iodideRegulated cell death

Identifiers

PMID41811524
PMCPMC12979305

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.