Evidence map›Paper›PMID 41822567›Full record

ArticleVeterinary world2026

Effect of direct-fed microbial feed blocks on blood β-hydroxybutyrate and milk yield in early postpartum buffaloes under field conditions.

Duggirala Srinivas Murty, Vishal Suthar, Bhavinaben Manharbhai Rathva, Manasvi Bhikhabhai Ladola, Aarti Bipinbhai Desai, Raj Desai, Deepak B Patil, Paresh Pandya

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Article in Veterinary world, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Duggirala Srinivas MurtyDepartment of Microbiology, Gujarat Vidyapith, Ahmedabad- 380014, Gujarat, India.
Vishal SutharAnimal Biotechnology Division, Gujarat Biotechnology University, GIFT City, Gandhinagar-382355, Gujarat, India.
Bhavinaben Manharbhai RathvaDepartment of Microbiology, Gujarat Vidyapith, Ahmedabad- 380014, Gujarat, India.
Manasvi Bhikhabhai LadolaDepartment of Microbiology, Gujarat Vidyapith, Ahmedabad- 380014, Gujarat, India.
Aarti Bipinbhai DesaiDepartment of Microbiology, Gujarat Vidyapith, Ahmedabad- 380014, Gujarat, India.
Raj DesaiDirectorate of Research, Kamdhenu University, Gandhinagar-382010, Gujarat, India.
Deepak B PatilDirectorate of Research, Kamdhenu University, Gandhinagar-382010, Gujarat, India.
Paresh PandyaAnimal Nutrition Research Station, Anand-388001, Gujarat, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: Early postpartum buffaloes are highly susceptible to negative energy balance and hyperketonemia, which adversely affect metabolic health, milk yield, and farm profitability under smallholder field conditions. Direct-fed microbials (DFMs) have emerged as a promising nutritional strategy to improve rumen function and productivity; however, evidence in buffaloes under real-farm settings remains limited. This study evaluated the effect of DFM feed blocks on blood β-hydroxybutyrate (BHB) concentrations and milk yield in early postpartum buffaloes managed at farmers' doorsteps. Materials and Methods: A field-based randomized controlled trial was conducted between February and December 2023 in three dairy herds in Gandhinagar district, Gujarat, India. Initially, 36 early postpartum buffaloes were enrolled; due to attrition, 22 animals (11 per group) were included in the final analysis. Buffaloes were randomly allocated to a DFM group or a placebo group. The DFM group received feed blocks containing a consortium of probiotic strains cultivated on agricultural by-products, while the placebo group received wheat straw blocks, both alongside a nutritionally balanced basal diet. The intervention lasted five weeks. Blood BHB concentrations were measured weekly on days 0, 7, 14, 21, and 28 using a validated handheld meter, and daily milk yield was recorded throughout the study. Data were analyzed using linear mixed models with repeated-measures. Results: DFM supplementation significantly reduced mean blood BHB concentrations compared with the placebo (1.04 ± 0.04 vs. 1.40 ± 0.03 mmol/L; p < 0.05), indicating improved metabolic status. A significant treatment × time interaction demonstrated a progressive decline in BHB levels from day 7 onward in the DFM group. Buffaloes receiving DFM produced significantly more milk than controls (9.27 ± 2.91 vs. 7.35 ± 0.31 L/day), corresponding to an average increase of 1.73 ± 0.42 L/day (p < 0.001), with consistent effects across the experimental period. Conclusion: Under practical field conditions, DFM feed blocks effectively improved metabolic health by lowering blood BHB concentrations and significantly enhanced milk production in early postpartum buffaloes. These findings support the use of DFMs as a sustainable nutritional intervention for improving productivity and metabolic resilience in buffalo-based dairy systems, while highlighting the need for further mechanistic and long-term studies.

Indexed as

beta-hydroxybutyratebuffalodirect-fed microbialsearly postpartumhyperketonemiametabolic healthmilk yieldprobiotic feed

Identifiers

PMID41822567
PMCPMC12975729

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.