Evidence map›Paper›PMID 41824448›Full record

ArticleSTAR protocols2026

Protocol for the quantification of digestive exophagy in cell culture.

Lucy Funes, Frederick R Maxfield, Santiago Solé-Domènech

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Lucy FunesDepartment of Biochemistry and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA.
Frederick R MaxfieldDepartment of Biochemistry and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: mfrmaxfie@med.cornell.edu.
Santiago Solé-DomènechDepartment of Biochemistry and Biophysics, Weill Cornell Medicine, New York, NY 10065, USA. Electronic address: sas2068@med.cornell.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microglia use digestive exophagy to partially degrade, extracellularly, Alzheimer's amyloid-beta aggregates that are too large to be phagocytosed. Here, we present a protocol to quantify this mechanism in cell culture. We describe steps for extracting primary microglial cells and preparing amyloid-beta model aggregates. We then detail procedures for measuring lysosomal exocytosis toward, and extracellular degradation of, these deposits using quantitative fluorescence microscopy. We also provide guidance on quantifying the data using digital image analysis. For complete details on the use and execution of this protocol, please refer to Jacquet et al.

Indexed as

Cell Culture TechniquesExocytosisMicrogliaPhagocytosisAmyloid beta-PeptidesAnimalsCells, CulturedMiceMicroscopy, FluorescenceAmyloid beta-PeptidesCell-based assaysCell biologyCell cultureCell isolationMicroscopyMolecular/chemical probesNeuroscience

Identifiers

PMID41824448
PMCPMC12997214

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.