Evidence map›Paper›PMID 41825443›Full record

ArticleNeuron2026

Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.

Colleen N McLaughlin, Hui Ji, Katherine X Dong, Chuanyun Xu, Kenneth Kin Lam Wong, Zhuoran Li, David J Luginbuhl, Charles Xu, Cheng Lyu, Wei Qin and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Colleen N McLaughlinDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: cnm@stanford.edu.
Hui JiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Katherine X DongDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Chuanyun XuDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Kenneth Kin Lam WongDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Zhuoran LiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
David J LuginbuhlDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Charles XuBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cheng LyuDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Wei QinDepartment of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Jiefu LiJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Namrata D UdeshiBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Steven A CarrBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Alice Y TingDepartment of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Liqun LuoDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu.

Funding

Assembly of the Central Olfactory Networks in DrosophilaR01DC005982 · NIDCD · STANFORD UNIVERSITY · PI LIQUN LUO · 2003 to 2026
$8.1M
Cell surface protein dynamics in neural circuit assemblyK99DC021195 · NIDCD · STANFORD UNIVERSITY · PI MCLAUGHLIN, COLLEEN · 2024 to 2025
$257k
NIDCD NIH HHS K99 DC021195NIDCD NIH HHS R01 DC005982
6 · The paper itself

Abstract

Endocytosis actively remodels the neuronal surface proteome to drive diverse cellular processes, yet its global extent and effects on neural circuit development have defied comprehensive interrogation. Here, we introduce endocytome profiling: a systematic, cell-type-specific approach for mapping cell-surface protein (CSP) dynamics in situ. Quantitative proteomic analysis of developing Drosophila olfactory receptor neuron (ORN) axons generated an endocytic atlas comprising over 1,000 proteins and revealed the extent to which the cell-surface proteome is remodeled to meet developmental demands. Targeted interrogation of a junctional CSP showed that its endosome-to-surface ratio is precisely balanced to enable developmental axon pruning while preserving mature axon integrity. Multi-omic integration uncovered widespread transcellular signaling and identified a growth factor secreted by neighboring neurons to direct ORN axon targeting via endocytic regulation of its receptor. Endocytome profiling provides unprecedented access to cell-surface proteome dynamics and offers a platform to dissect proteome-scale remodeling across diverse cell types and contexts.

Indexed as

Drosophila ProteinsEndocytosisMembrane ProteinsOlfactory Receptor NeuronsAnimalsAxonsDrosophilaDrosophila melanogasterEndosomesNeurodevelopmentProteomeProteomicsDrosophila ProteinsMembrane ProteinsProteomeaxon targetingcell-surface proteinDrosophilaendosomeolfactory receptor neuronproteomicsproximity labelingremodeling

Identifiers

PMID41825443
PMCPMC13055848

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.