Evidence map›Paper›PMID 41826945›Full record

ArticleBMC biotechnology2026

p53 mediated cell cytotoxicity and DNA damage in lung cancer cell line A549 triggered by a small peptide extracted from a probiotic strain Lacticaseibacillus casei.

Jannatul Firdous Siddique, G R Shree Kumari, Sudarshana Deepa Vijay Kumar, C Subathra Devi, Mohanasrinivasan Vaithilingam

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jannatul Firdous SiddiqueSchool of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India.ORCID 0000-0003-3085-3357
G R Shree KumariSchool of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India.ORCID 0000-0003-4833-1856
Sudarshana Deepa Vijay KumarDepartment of Biotechnology, National Institute of Technology, Tadepalligudem, Andhra Pradesh, 534101, India.
C Subathra DeviSchool of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India.ORCID 0000-0002-1828-8212
Mohanasrinivasan VaithilingamSchool of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India. v.mohan@vit.ac.in.ORCID 0000-0001-7638-351X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAntimicrobial peptides are being explored as anticancer agents due to their selectivity toward transformed cells. We evaluated a small peptide from Lacticaseibacillus casei for anti-proliferative effects on A549 lung cancer cells and investigated apoptosis-related mechanisms in vitro.

methodsCell viability was assessed by MTT. Apoptosis-associated morphology was examined by AO/EB staining, DNA fragmentation by agarose gel electrophoresis, and gene expression by qRT-PCR (p53, BAX, BCL-2, CASP8, CASP7, CASP3).

resultsThe peptide reduced A549 viability in a dose-dependent manner relative to L929 cells. AO/EB staining revealed early apoptotic nuclear morphology, and DNA fragmentation was observed. qRT-PCR showed increased p53 and BAX, increased CASP8, downregulation of CASP7, and no significant change in CASP3.

conclusionsData support apoptosis that is predominantly p53/BAX-driven with limited effector caspase engagement. As per experimental evidence of the study, the peptide from Lacticaseibacillus casei has the potential to inhibit the proliferation of Lung cancer cell line A549.

Indexed as

Antineoplastic AgentsDNA DamageLacticaseibacillus caseiLung NeoplasmsProbioticsTumor Suppressor Protein p53A549 CellsApoptosisbcl-2-Associated X ProteinCaspase 3Caspase 8Cell ProliferationCell SurvivalHumansAntineoplastic Agentsbcl-2-Associated X ProteinCaspase 3Caspase 8TP53 protein, humanTumor Suppressor Protein p53ApoptosisCaspaseCytotoxicLacticaseibacillis caseiLung cancerPeptide

Identifiers

PMID41826945
PMCPMC13101141

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.