Evidence map›Paper›PMID 41828659›Full record

ArticleInternational journal of molecular sciences2026

Integrative Analysis of VSMC, Macrophage, and Fibroblast Responses to LDLs in Aortic Pathologies.

Ulyana Khovantseva, Diana Kiseleva, Vadim Cherednichenko, Denis Breshenkov, Diana Matveeva, Tatiana Kirichenko, Yuliya Markina, Eduard Charchyan, Alexander Markin

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ulyana KhovantsevaPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-2875-6999
Diana KiselevaPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0001-8149-349X
Vadim CherednichenkoPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-1695-2060
Denis BreshenkovPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-9459-9282
Diana MatveevaInstitute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia.ORCID 0000-0002-1386-2836
Tatiana KirichenkoPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-2899-9202
Yuliya MarkinaPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-3781-6340
Eduard CharchyanPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-0488-2560
Alexander MarkinPetrovsky National Research Center of Surgery, 119435 Moscow, Russia.ORCID 0000-0002-6649-7924

Funding

The Ministry of Science and Higher Education of the Russian Federation FURG-2026-0032
6 · The paper itself

Abstract

Cardiovascular diseases (CVDs) remain the leading cause of global mortality, with aortic pathologies such as atherosclerosis and thoracic aortic aneurysm posing significant risks due to their asymptomatic nature and potential fatal complications. This study investigates molecular mechanisms underlying CVDs by examining key cellular components of the aortic wall-vascular smooth muscle cells (VSMCs), fibroblasts, and macrophages-and their responses to low-density lipoproteins (LDLs). Using in vitro models, we analyzed phenotypic characteristics, LDL internalization capacity, and secretion/expression of pro-inflammatory mediators (IL-6, IL-8, IL-1β, CCL2) in primary VSMCs (from tunica intima and media), fibroblasts (977hTERT), and THP-1 macrophages. Fluorescence staining with BDP 630/650 revealed that all cell types internalize LDLs, with macrophages showing the highest lipid accumulation. ELISA and RT-qPCR demonstrated cell-specific patterns of cytokine secretion and gene expression, both in control conditions and after LDL exposure. The results indicate that VSMCs and fibroblasts, normally involved in vascular tone maintenance and extracellular matrix (ECM) synthesis, acquire pro-inflammatory features under pathological conditions, including increased secretion of IL-6, IL-8, and CCL2. Macrophages exhibited enhanced expression of the scavenger receptor CD36 and pro-inflammatory cytokines (especially IL-1β) after LDL treatment.

Indexed as

AortaFibroblastsLipoproteins, LDLMacrophagesMuscle, Smooth, VascularMyocytes, Smooth MuscleAtherosclerosisCells, CulturedCytokinesHumansCytokinesLipoproteins, LDLcardiovascular diseasesfibroblastslow-density lipoproteinsmacrophagesvascular smooth muscle cells

Identifiers

PMID41828659
PMCPMC12986006

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.