Tissue-Based Transcriptomic Profiling of Gastrointestinal Graft Versus Host Disease Reveals Immune and MicroRNA Dysregulation.

Sakhila Ghimire, Jean Norden, Rihab Gam, Clare Lendrem, Ernst Holler, Anne M Dickinson, Rachel E Crossland

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sakhila GhimireDepartment of Internal Medicine III, University Medical Centre, 93053 Regensburg, Germany.

Jean NordenTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Rihab GamTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Clare LendremTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.ORCID 0000-0002-9435-7398

Ernst HollerDepartment of Internal Medicine III, University Medical Centre, 93053 Regensburg, Germany.

Anne M DickinsonTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.

Rachel E CrosslandTranslational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.ORCID 0000-0001-6138-160X

Funding

Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) 324392634Wellcome Trust 315963Wilhelm Sander Stiftung 2022.116.1

6 · The paper itself

Abstract

Gastrointestinal acute graft-versus-host disease (GI aGvHD) remains a leading cause of non-relapse mortality after allogeneic hematopoietic stem cell transplantation (HSCT). Current diagnostic methods rely on invasive procedures with limited sensitivity. While circulating biomarkers have been proposed, little is known about the local transcriptomic landscape within inflamed GI tissue. We performed integrated profiling of mRNA and microRNA expression in colonoscopically resected GI biopsies from n = 8 HSCT patients, including n = 3 with histologically confirmed GI aGvHD and n = 5 without. Using NanoString nCounter technology, we quantified 770 immune-related mRNAs and 799 mature human microRNAs. Differential expression analysis, pathway enrichment, cell type deconvolution, and machine learning-based biomarker prioritisation were conducted to define disease-specific molecular signatures. GI aGvHD was marked by upregulation of inflammatory genes (e.g.,

Indexed as

Gastrointestinal DiseasesGene Expression ProfilingGraft vs Host DiseaseMicroRNAsTranscriptomeAdultBiomarkersFemaleGene Expression RegulationHematopoietic Stem Cell TransplantationHumansMaleMiddle AgedRNA, MessengerBiomarkersMicroRNAsRNA, MessengerGvHDHSCTMicroRNAmolecular profilingtransplantation

Identifiers

PMID41828729

DOI10.3390/ijms27052513

PMCPMC12986492

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.