Evidence mapPaperPMID 41833110Full record

ArticlePoultry science2026

Dietary semen cuscuta improves laying performance by stabilizing mitochondria-associated membranes (MAMs) and inhibiting granulosa cell apoptosis in laying hens.

Changqing Si, Ying Chen, Linjie Liu, Shuo Liu, Zhenbiao Zhang, Lihuan Guo, Mengpo Zhao, Yuming Chen, Yuxiang Shi, Wanyu Shi and 1 more

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Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Changqing SiSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China.
Ying ChenBethune International Peace Hospital, Shijiazhuang 050000, China.
Linjie LiuSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China.
Shuo LiuSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China.
Zhenbiao ZhangShanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, 030801, China.
Lihuan GuoHebei Kangli Animal Pharmaceutical Co., Ltd., Shijiazhuang 050000, China.
Mengpo ZhaoSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China.
Yuming ChenSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China. Electronic address: chenyuming@hebeu.edu.cn.
Yuxiang ShiSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China. Electronic address: hbyxshi@126.com.
Wanyu ShiCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, 071000, China. Electronic address: shiwanyu@hebau.edu.com.
Shuang MaSchool of Life Science and Food Engineering, Hebei University of Engineering, Handan 056000, China. Electronic address: mashuang@hebeu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The decline in laying performance during late-phase production of hens, primarily due to ovarian aging, poses significant economic challenges to the poultry industry. We have previously identified that Semen Cuscuta (SC), a traditional Chinese herbal medicine, could alleviate reproductive damage in mice, however, the therapeutic potential of SC in aging hens remains largely unexplored. In this study, different doses of SC (1%, 1.5%, or 2%) were dietary supplemented for 4 weeks, and serum, Ileum, ovarian tissues were collected for further detection/examination. Results showed that 1.5% and 2% SC significantly improved laying performance, enhanced systemic antioxidant capacity (increased serum SOD, decreased MDA), and elevated FSH levels. SC also reinforced intestinal barrier integrity, as indicated by improved villus morphology, upregulation of tight junction proteins (Occludin, ZO-1), and reduced serum endotoxin markers (D-LA, LPS). Moreover, SC modulated gut microbiota composition, increased cecal short-chain fatty acids (acetic and butyric acid), and upregulated intestinal specifically short-chain fatty acids (SCFA) receptors (GPR41, GPR43). In the ovary, SC suppressed granulosa cell apoptosis by modulating Bcl-2, Bax, Cleaved Caspase-3, and Cyt c expression. Notably, transmission electron microscopy revealed that SC preserved mitochondrial associated membrane (MAM) structural integrity and normalized the expression of MAM tethering proteins (IP3R, GRP75, VDAC1) in ovary. These findings demonstrate that SC ameliorates ovarian aging and restores laying performance through a dual mechanism: directly stabilizing ovarian MAMs to inhibit apoptosis, and indirectly remodeling gut microbiota to enhance intestinal barrier function and reduce systemic inflammation. This study provides a mechanistic basis for SC as a promising feed additive to improve sustainability in the production of aging hens.

Indexed as

ApoptosisChickensCuscutaDrugs, Chinese HerbalGranulosa CellsReproductionAnimal FeedAnimalsDietDietary SupplementsDose-Response Relationship, DrugFemaleMitochondriaRandom AllocationDrugs, Chinese HerbalAntioxidant capacityGut microbiotaMAMsOvarian agingSemen Cuscuta (SC)

Identifiers

PMID41833110
PMCPMC13000509

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.