Evidence map›Paper›PMID 41833116›Full record

ArticlePoultry science2026

Stage-dependent effects of composite probiotics on gut mineral transport, Skeletal health, and egg quality in laying hens.

Jiajun Zhu, Zhi Hu, Hengyong Xu, Jiapeng Chen, Xuyang Ji, Xinqi Wang, Longge Guo, Guoqing Zhang, Ting Yang, Shiyu Yang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jiajun ZhuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Zhi HuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Hengyong XuState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Jiapeng ChenState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Xuyang JiState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Xinqi WangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Longge GuoState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Guoqing ZhangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Ting YangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Shiyu YangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China.
Yan WangState Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, PR China, Chengdu 611130;; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, PR China;; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, PR China. Electronic address: as519723614@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two hundred and forty one-day-old Lohmann Brown laying hens were used to investigate the stage-dependent effects of dietary supplementation with a composite probiotic on production performance, egg quality, calcium and phosphorus metabolism, skeletal characteristics, and the expression of mineral transport- and bone metabolism-related genes from 1 to 52 weeks of age. Hens were randomly allocated to four dietary treatments with six replicates of ten birds per replicate. The treatments differed in the timing of probiotic supplementation relative to the onset of lay, including continuous supplementation, early-life supplementation only, late-phase supplementation only, and no supplementation throughout the experiment. The composite probiotic consisted of Bacillus subtilis, lactic acid bacteria, and yeast. During the peak laying period, hens receiving probiotics showed higher egg production rates and lower eggshell breakage (P < 0.05). In the late laying period, continuous probiotic supplementation further reduced eggshell breakage and maintained higher laying performance compared with non-supplemented hens (P < 0.05). Egg quality was improved by probiotic inclusion, as indicated by increased eggshell strength and yolk height during peak and late lay (P < 0.05), although eggshell thickness was slightly reduced (P < 0.05).Probiotic supplementation exerted marked effects on skeletal traits and mineral metabolism in a stage-dependent manner. Continuous supplementation increased tibial calcium and phosphorus content during the peak and late laying periods (P < 0.05) and alleviated age-related reductions in bone mineral density. At the molecular level, probiotic-fed hens exhibited increased intestinal expression of calcium and phosphorus transport-related genes, including TRPV6, PMCA1b and NaPi-IIb, particularly during peak and late laying stages (P < 0.05). In tibial tissue, continuous probiotic supplementation enhanced the expression of osteogenic markers, including RUNX2 (P < 0.05).In conclusion, composite probiotic supplementation produces stage-dependent improvements in mineral transport, bone health, and egg quality in laying hens. Continuous supplementation throughout the production cycle is most effective for maintaining skeletal mineral reserves and reducing egg breakage, providing a practical nutritional strategy for long-cycle commercial laying hens.

Indexed as

ChickensMineralsOvumProbioticsAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsBone and BonesDietDietary SupplementsEgg ShellFemaleRandom AllocationReproductionMineralsCalcium-phosphorus transportEgg qualityLaying hensProbioticsProduction performanceSkeletal system

Identifiers

PMID41833116
PMCPMC13000498

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.