Evidence map›Paper›PMID 41834725›Full record

ArticleCirculation research2026

RHOT Proteins Link Mitochondrial Motility to Cardiomyocyte Sarcomere Maturation.

Natali Froese, Ivanna Shymotiuk, Alexander Froese, Felix Polten, Jonas Jablinski, Tim Scholz, Mortimer Korf-Klingebiel, Christopher Werlein, Paolo Galuppo, Anna Gigina and 19 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Circulation research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Peripartum-Associated Heart Failure Develops Independently of RHOT Proteins.International journal of molecular sciences · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

29 authors.

Natali FroeseDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.
Ivanna ShymotiukDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0009-0007-8513-1606
Alexander FroeseInstitute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf, Germany (A.F., V.O.N.).
Felix PoltenDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0002-1076-5272
Jonas JablinskiDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.
Tim ScholzInstitute of Molecular and Cell Physiology (T. Scholz), Hannover Medical School, Germany.ORCID 0000-0002-2127-9843
Mortimer Korf-KlingebielDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0002-4388-2117
Christopher WerleinInstitute of Pathology (C. Werlein, M.P.K., D.D.J.), Hannover Medical School, Germany.ORCID 0000-0002-7694-4257
Paolo GaluppoDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0001-6745-5681
Anna GiginaDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.
Katharina WihlerDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.
Johanna SchneiderMedical Center-University of Freiburg, Germany (J.S.).ORCID 0000-0003-0661-6984
Sergej ErschowDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0009-0004-9586-0202
Maren HeimerlDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.
Malgorzata SzaroszykDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0009-0008-4051-3958
Jan HegermannInstitute of Functional and Applied Anatomy, Research Core Unit Electron Microscopy (J.H., C. Wrede), Hannover Medical School, Germany.ORCID 0000-0001-7893-8507
Christoph WredeInstitute of Functional and Applied Anatomy, Research Core Unit Electron Microscopy (J.H., C. Wrede), Hannover Medical School, Germany.ORCID 0000-0001-7461-6965
Theresa SchweitzerInstitute of Toxicology, Research Core Facility Proteomics, (T. Schweitzer, A.P.), Hannover Medical School, Germany.
Andreas PichInstitute of Toxicology, Research Core Facility Proteomics, (T. Schweitzer, A.P.), Hannover Medical School, Germany.ORCID 0000-0001-8444-0940
Robert GeffersHelmholtz Center for Infection Research, Research Group Genome Analytics, Braunschweig, Germany (R.G.).ORCID 0000-0003-4409-016X
Melanie Ricke-HochDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0001-5479-2275
Mark P KühnelInstitute of Pathology (C. Werlein, M.P.K., D.D.J.), Hannover Medical School, Germany.ORCID 0000-0003-3558-2576
Danny D JonigkInstitute of Pathology (C. Werlein, M.P.K., D.D.J.), Hannover Medical School, Germany.ORCID 0000-0002-5251-2281
Adam R WendeDivision of Molecular and Cellular Pathology, Department of Pathology, University of Alabama at Birmingham (A.R.W.).ORCID 0000-0002-5536-4675
E Dale AbelDavid Geffen School of Medicine and UCLA Health (E.D.A.).ORCID 0000-0001-5290-0738
Viacheslav O NikolaevInstitute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf, Germany (A.F., V.O.N.).ORCID 0000-0002-7529-5179
Kai C WollertDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0002-4890-4450
Johann BauersachsDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0002-9341-117X
Christian RiehleDepartment of Cardiology and Angiology (N.F., I.S., F.P., J.J., M.K.-K., P.G., A.G., K.W., S.E., M.H., M.S., M.R.-H., K.C.W., J.B., C.R.), Hannover Medical School, Germany.ORCID 0000-0002-0040-4254

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCardiomyocyte mitochondria align with sarcomeres during heart development. Mitochondrial motility is controlled by RHOT (ras homolog family member T) 1 and RHOT2. RHOT1 and RHOT2 are atypical Rho-like small GTPases that are anchored to the outer mitochondrial membrane and couple mitochondria to kinesin and dynein motors. We hypothesized that RHOT protein expression and mitochondrial motility are required for mitochondrial positioning during cardiomyocyte development.

methodsWe generated mice with cardiomyocyte-selective deletion of

resultscRhot1/2-KO mice developed fatal cardiomyopathy associated with sarcomere disarray and perinuclear accumulation of mitochondria and ATP production. Mitochondria isolated from cRhot1/2-KO hearts exhibited impaired motility but preserved respiratory capacity. Mechanistically, proteome analysis identified that RHOT proteins bind mitochondria to contractile muscle fiber proteins. In contrast, inducible deletion of

conclusionsRHOT proteins bind mitochondria to contractile muscle fiber proteins and are required for mitochondrial positioning in cardiomyocytes during development. Our study links mitochondrial motility and local ATP production to structural and functional maturation of the heart.

Indexed as

Mitochondria, HeartMyocytes, Cardiacrho GTP-Binding ProteinsSarcomeresAdenosine TriphosphateAnimalsCells, CulturedMiceMice, KnockoutAdenosine Triphosphaterho GTP-Binding Proteinsheart failuremitochondriamyocytes, cardiacproteomesarcomere

Identifiers

PMID41834725
PMCPMC13108437

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.