ArticleFrontiers in plant science2026
Construction and application of multiple nucleotide polymorphism-based DNA fingerprinting for
Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Methods: We successfully developed a high-throughput identification system based on multiple nucleotide polymorphism (MNP) markers for Results: Validation results revealed that this marker panel had excellent amplification efficiency and polymorphism across all 80 tested accessions. For the MNP markers, the average number of allelic genotypes reached 28.95 ± 15.11. The polymorphism information content was 0.73 ± 0.16. Both metrics were substantially superior to those of traditional identification methods. Both phylogenetic analysis and principal component analysis distinguished all the tested accessions, with an identification accuracy rate of 99.90%. A unique molecular ID code was assigned to each cultivar. Discussion: The MNP marker system developed in this study combines the advantages of high throughput, high accuracy, and favorable reproducibility. It provides the technical means and solutions for the authenticity identification of
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