ArticleTranslational animal science2026
Evaluation of milk-based and egg-based semen extenders for extended storage of ram semen at 4°C.
Article in Translational animal science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ram semen cryopreservation and liquid storage are essential tools for reproductive management in sheep, but remain limited by the sensitivity of sperm to cold shock and oxidative damage. Commercial extenders aim to preserve sperm function, yet differences in biochemical composition may influence their efficacy across key indicators of fertility. This study evaluated the effects of two commercial extenders, INRA96 and OviPlus, on ram sperm quality during liquid storage at 4°C over a 96-h period. Semen samples were collected from mature rams and diluted into each extender, then assessed at 0, 12, 24, 36, 48, 60, and 96 h post-collection for membrane integrity, acrosomal remodeling, reactive oxygen species (ROS), and zinc signatures using image-based flow cytometry (IBFC), as well as motility parameters measured with Computer Assisted Semen Analysis (CASA). While both extenders supported initial sperm viability, OviPlus had significantly less plasma membrane destabilization (timepoints 0, 12, 36, 48;
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