ArticleArchives of microbiology2026
Promoter-driven enhancement of PHBV synthesis in recombinant Escherichia coli using glucose and propionate as carbon sources.
Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors.
Funding
Abstract
Polyhydroxyalkanoates (PHAs) are promising biodegradable polymers synthesized by bacteria, yet their widespread application remains limited by production cost and yield. To achieve cost-effective and efficient production of PHA, PHA synthesis-related genes (phaCR and phaABR) from Rhodoligotrophos defluvii lm1T were cloned into plasmids under different promoters (Ptac, PCn, and lac-tac) and expressed in Escherichia coli DH5α with a ptsG gene knockout to relieve carbon catabolite repression in this study. Among the recombinant strains, L3/ΔptsG, containing the PCn promoter, achieved the highest PHA yield (36.25 wt%) using 27 g/L glucose and 1.5 g/L sodium propionate as carbon sources. The produced polymer was identified as poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) by gas chromatography and nuclear magnetic resonance spectroscopy, exhibiting favorable monomer composition, molecular weight, and thermal stability (Td5 = 271 ℃). These findings demonstrate that combining promoter optimization with dual carbon-source feeding enables efficient PHBV production in engineered E. coli, offering a viable route for cost-effective bioplastic synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.