Bacillus amyloliquefaciens induces autophagy-mediated defense, phytohormonal modulation, and metabolic reprogramming in Oryza sativa to counteract Rhizoctonia solani sheath blight.

key messageBacillus amyloliquefaciens (SN13) priming boosts rice resistance to sheath blight through activation of autophagy, regulation ofphytohormone signaling, and metabolic reprogramming. Sheath blight disease, caused by Rhizoctonia solani, poses a major threat to rice productivity worldwide. The present study examines how phytohormone signalling networks coordinate with SN13-mediated defense and reshape metabolic pathways during pathogen challenge. Glasshouse experiments revealed that SN13 inoculation markedly improved physiological resilience under R. solani infection, as evidenced by enhanced chlorophyll content, reduced lipid peroxidation, and lower accumulation of proline and total soluble sugars. Improved antioxidative capacity and efficient ROS detoxification was further confirmed through DAB and NBT staining. Gene expression profiling demonstrated substantial induction of autophagy-related and phytohormone-responsive genes, particularly under combined SN13-salicylic acid (SA) or SN13-jasmonic acid (JA) treatments. While pathogen infection alone suppressed autophagy and disrupted endogenous hormone balance, SN13 reinstated ATG gene expression, stabilized auxin, JA, and cytokinin signaling, and mitigated cellular damage. Collectively, these findings demonstrate that SN13 enhances rice tolerance to sheath blight by coordinating autophagy, phytohormonal cross-regulation, and metabolic reprogramming, thus offering a promising eco-friendly strategy for sustainable disease management.