Evidence map›Paper›PMID 41845515›Full record

ArticleMicrobiome2026

Limosilactobacillus reuteri enhances production performance and ovarian function via modulation of the gut-ovary axis in laying hens.

Yanchao Geng, Hui Wang, Fenglong Cai, Liang Hong, Lei Pu, Shunyi Qin, Hua Yang, Jianbin Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanchao GengTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Hui WangTianjin Guangyuan Livestock and Poultry Breeding Co., Ltd., Baodi District, Tianjin, 301800, China.
Fenglong CaiTianjin Guangyuan Livestock and Poultry Breeding Co., Ltd., Baodi District, Tianjin, 301800, China.
Liang HongTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Lei PuTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Shunyi QinTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Hua YangTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China.
Jianbin ZhangTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300392, China. zjbzwz@126.com.

Funding

the Enterprise Science and Technology Commissioner Project under Tianjin Special (Fund) for Technology Innovation Guidance 23YDTPJC00440the "Jiebang Guashuai" Project for Agricultural Industry-University-Research-Application Collaboration of Tianjin GBGG202427
6 · The paper itself

Abstract

backgroundDeclines in nutrient absorption and reproductive function following peak laying significantly compromise egg production and quality in hens. Bacterial and enzymatic co-fermented feed is a promising intervention to improve gut health and production metrics. However, its mechanism of action remains unclear.

resultsBacterial and enzymatic co-fermented feed supplementation in laying hens significantly enhanced egg production, egg weight, and feed intake while reducing the feed conversion ratio. Egg quality improved markedly, denoted by an increase in albumen height, the Haugh unit, and eggshell strength and thickness. The apparent digestibility of calcium in laying hens increased. Although the tibial growth indices remained unchanged, bone microarchitecture improved, evidenced by increased trabecular mineralisation and a reduction in the number of osteoclasts. Intestinal integrity was strengthened based on an elevated villus/crypt ratio, upregulation of barrier genes (ZO-1, Claudin-1, Occludin), increased IL-10 expression, and decreased IL-6 and TNF-α expression. Bacterial and enzymatic co-fermented feed reshaped the caecal microbiota, enriching Limosilactobacillus. This genus was also enriched in the oviduct and ovary. SourceTracker analysis revealed a caecum-oviduct-ovary microbial transmission route. In the ovary, bacterial and enzymatic co-fermented feed enhanced the expression of FSHR, AMH, and antioxidant genes (Nrf2, SOD1, CAT, GPX3, and HO-1); upregulated Bcl-2; and downregulated Caspase-3 and Caspase-8, supporting improved follicular health and antioxidant defences.

conclusionsThis study is the first to clarify the regulatory mechanism of bacterial and enzymatic co-fermented feed on laying performance of laying hens from a microbial perspective. The co-fermented feed improved the laying performance, egg quality and tibial microarchitecture of laying hens by optimizing the microbial composition of the cecum, oviduct and ovary, thus delaying ovarian aging and enhancing ovarian function. Furthermore, we found the ovarian microbial composition of laying hens was affected by the oviduct microbiota, which was in turn influenced by the intestinal and cloacal microbiota, providing experimental evidence for the gut-oviduct-ovary axis in laying hens and a new perspective for exploring the correlation between feed nutrition and reproductive performance of laying hens. Video abstract.

Indexed as

Animal FeedChickensGastrointestinal MicrobiomeOvaryAnimalsCecumEggsFemaleFermentationOviductsProbioticsReproductionFermented feedGut-ovary axisLaying hensLaying performanceOvarian function

Identifiers

PMID41845515
PMCPMC13107617

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.