Evidence mapPaperPMID 41857221Full record

ArticleScientific reports2026

Effect of molecular weight, temperature, and pH on antioxidant activity of whey protein isolate hydrolysate and its application in pasta fortification.

Melika Mohammadi, Hassan Ahmadi Gavlighi, Roghayeh Amini Sarteshnizi, Amir Pouya Ghandehari Yazdi, Amin Karimi

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Melika MohammadiDepartment of Food Science and Technology, Tarbiat Modares University, Tehran, Iran.
Hassan Ahmadi GavlighiDepartment of Food Science and Technology, Tarbiat Modares University, Tehran, Iran. Ahmadi_ha@modares.ac.ir.
Roghayeh Amini SarteshniziDepartment of Food Science and Technology, Tarbiat Modares University, Tehran, Iran.
Amir Pouya Ghandehari YazdiDepartment of Research and Development, Zarmacaron Company, Zar Industrial and Research Group, Alborz, Iran.
Amin KarimiDepartment of Food Science and Technology, Tarbiat Modares University, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study explored the enzymatic hydrolysis of whey protein concentrate (WPC) and isolate (WPI), and evaluated the antioxidant, physicochemical, textural, and sensory properties of pasta fortified with whey protein hydrolysate (WPH). WPI hydrolysate (WPIH) exhibited higher antioxidant activity than WPC hydrolysate (WPCH), with DPPH and ABTS⁺ radical scavenging activity (RSA) values of 6 and 211 µmol Trolox/g dry weight, respectively, at 5% enzyme to substrate (E/S). Fractionation of WPIH revealed that peptides lower than 2 KDa (F1) had the highest ABTS⁺ RSA (237 µmol Trolox/g), while peptides higher than 10 KDa (F3) showed the greatest DPPH RSA (6 µmol Trolox/g) and Fe²⁺ chelating capacity (13 µmol EDTA/g). Antioxidant stability was highest at pH 5 and 60 °C. Incorporation of 0.5–1.5 g/100 g WPIH into pasta significantly enhanced antioxidant properties, increasing DPPH RSA from 0.89 to 2 µmol Trolox/g, ABTS⁺ RSA from 136 to 463 µmol Trolox/g, and Fe²⁺ chelation from 6.8 to 8.5 µmol EDTA/g. Fortified pasta also showed higher protein, reduced cooking time, increased cooking loss, and decreased hardness. Sensory evaluation indicated acceptable quality up to 1 g/100 g WPIH, beyond which texture and aroma declined. These findings suggest that WPIH can be used as a functional component in pasta to increase nutritional value and antioxidant capacity at modest inclusion levels without lowering consumer acceptability.

Indexed as

AntioxidantsFood, FortifiedProtein HydrolysatesWhey ProteinsFood, ProcessedHydrogen-Ion ConcentrationHydrolysisMolecular WeightTemperatureAntioxidantsProtein HydrolysatesWhey ProteinsAntioxidant activityFunctional pastaUltrafiltrationWhey protein hydrolysate

Identifiers

PMID41857221
PMCPMC13139492

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.