Evidence map›Paper›PMID 41860621›Full record

ArticleMolecular biology reports2026

AAV-mediated surtuin 1 overexpression in the cochlear inner hair cells protects ribbon synapses of D-galactose-induced aging mice.

Zijing Yang, Zihui Chen, Chunli Zhao, Shusheng Gong, Zhengde Du, Shuguang Han

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Zijing YangDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China.
Zihui ChenDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China.
Chunli ZhaoDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China.
Shusheng GongDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China. gongss@ccmu.edu.cn.
Zhengde DuDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China. duzhengde@ccmu.edu.cn.
Shuguang HanDepartment of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, No.95, Yong'an Road, Xicheng District, Beijing, 100050, China. chris_hsg@126.com.

Funding

National Natural Science Foundation of China 82171132National Natural Science Foundation of China 82401360
6 · The paper itself

Abstract

backgroundMitochondrial dysfunction is considered one of the molecular mechanisms underlying age-related hearing loss (ARHL). While some studies have suggested that surtuin 1 (SIRT1) may improve mitochondrial function, delaying the onset of ARHL; other research has indicated that SIRT1 can exacerbate hair cell damage, leading to further hearing loss. We aimed to investigate the role of SIRT1 in ARHL and its associated molecular mechanisms.

methodsEight-week-old male C57BL/6J mice were randomly divided into three groups: control, D-galactose (D-gal), and D-gal+SIRT1. Auditory brainstem response (ABR) was tested to evaluate hearing function. Immunofluorescence (IF) staining was used to assess the number of ribbon synapses. The enzymatic chemistry was applied for measuring reactive oxygen species (ROS) production, and mitochondrial DNA (mtDNA) damage was assessed with RT-PCR. Additionally, western blot (WB) was used to detect SIRT1 protein levels.

resultsCompared with the control group, the D-gal group exhibited elevated ABR thresholds and decreased ABR I wave amplitude, as well as prolonged latency. The results also revealed a decrease in the number of ribbon synapses and increased ROS levels in the D-gal group, as well as decreased SIRT1 protein. SIRT1 overexpression mitigated hearing loss in D-gal-induced accelerated aging mice, with increased ABR I wave amplitude and shorter latency. WB analysis revealed that SIRT1 overexpression reduced total protein acetylation levels. Furthermore, SIRT1 overexpression decreased ROS production, mitigated mtDNA damage and significantly increased the number of synapses.

conclusionsThese findings demonstrate that SIRT1 overexpression suppresses oxidative stress in ARHL, thereby mitigating damage to inner hair cell ribbons and delaying the hearing deterioration in ARHL.

Indexed as

AgingHair Cells, Auditory, InnerPresbycusisSirtuin 1AnimalsDependovirusDNA, MitochondrialEvoked Potentials, Auditory, Brain StemGalactoseMaleMiceMice, Inbred C57BLMitochondriaReactive Oxygen SpeciesSynapsesDNA, MitochondrialGalactoseReactive Oxygen SpeciesSirt1 protein, mouseSirtuin 1ARHLInner hair cellMitochondriaROSSIRT1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.