Evidence map›Paper›PMID 41865378›Full record

ArticleSTAR protocols2026

Protocol to quantify cell-surface vimentin on senescent human chondrocytes and other cell types via a cell-based ELISA.

Jana Riegger, Hartmut Geiger

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jana RieggerDivision for Biochemistry of Joint and Connective Tissue Diseases, Department of Orthopedics, University of Ulm, 89081 Ulm, Germany. Electronic address: jana.riegger@uni-ulm.de.
Hartmut GeigerInstitute of Molecular Medicine, University of Ulm, 89081 Ulm, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surface markers for the identification and potential targeting of senescent cells are limited and typically assessed via flow cytometry. Here, we present a protocol to quantify cell-surface vimentin (CSV) as a membrane-associated marker of senescent chondrocytes using a cell-based ELISA. We describe the steps for primary chondrocyte culture and chemical induction of senescence. This protocol offers a high-throughput screening method to simultaneously quantify CSV across multiple samples with minimal cell input.

Indexed as

Cellular SenescenceChondrocytesVimentinBiomarkersCell MembraneCells, CulturedEnzyme-Linked Immunosorbent AssayFlow CytometryHumansBiomarkersVimentinCell cultureCell isolationCell MembraneFlow CytometryImmunologyProtein Biochemistry

Identifiers

PMID41865378
PMCPMC13309978

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.