Evidence map›Paper›PMID 41865519›Full record

ArticleFood chemistry2026

Conformation-sensitive nanobody immunoassay enables differentiation of live and heat-inactivated Salmonella Typhimurium for food safety monitoring.

Junkang Pan, Qiyi He, Zhihao Xu, Yijia Zhang, Wenkai Li, Jiayi Chen, Lulu Hu, Xiaolu Shi, Bruce D Hammock, Dongyang Li

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Junkang PanCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Qiyi HeCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Zhihao XuCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Yijia ZhangCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Wenkai LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Jiayi ChenCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China.
Lulu HuShenzhen Center for Disease Control and Prevention, Shenzhen 518055, People's Republic of China.
Xiaolu ShiShenzhen Center for Disease Control and Prevention, Shenzhen 518055, People's Republic of China.
Bruce D HammockDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, California 95616, United States.
Dongyang LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou 310058, People's Republic of China. Electronic address: dylee@zju.edu.cn.

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI YOUNG, THOMAS MICHAEL · 1987 to 2021
$50.1M
Supplement for bioactive lipids as effectors and indicators of the deleterious effects of human healthR35ES030443 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MORISSEAU, CHRISTOPHE HP · 2019 to 2025
$5.8M
NIEHS NIH HHS P42 ES004699NIEHS NIH HHS R35 ES030443
6 · The paper itself

Abstract

Salmonella Typhimurium remains a leading cause of foodborne gastroenteritis globally. However, verifying bacterial inactivation during food processing is hampered by the laborious nature of culture methods and the inability of current detection tools to discriminate viable from inactivated cells. This study presents a conformation-sensitive nanobody-based immunoassay that selectively recognizes heat-inactivated Salmonella Typhimurium. Nanobodies were generated via alpaca immunization and phage display, and two enzyme-linked immunosorbent assay (ELISA) were established: a direct ELISA and a sandwich ELISA enhanced by streptavidin-PolyHRP. The latter achieved a detection limit of 3.53 × 10

Indexed as

Salmonella typhimuriumSingle-Domain AntibodiesAnimalsEnzyme-Linked Immunosorbent AssayFood ContaminationFood MicrobiologyFood SafetyHot TemperatureSingle-Domain AntibodiesELISAHeat-inactivation treatmentNanobodyPolyHRPSalmonella Typhimurium

Identifiers

PMID41865519
PMCPMC13225312

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.