Evidence map›Paper›PMID 41868709›Full record

ArticleCytotechnology2026

Study on the effect of Ursolic acid on liver cancer cells using z-vad-fmk and ROS inhibitors.

Meng Xu, Jinyu Wu, Zhimin Huang, Mei Shi, Tingna Fu, Aitao Lin, Guoliang Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cytotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Meng XuGraduate School of Guangxi University of Traditional Chinese Medicine, No.13 Wuhe AvenueQingxiu District,, Nanning, 530000 , Guangxi China.
Jinyu WuThe First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, No. 89 - 9 Dongge Road,, Nanning, 530000 Guangxi China.
Zhimin HuangThe First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, No. 89 - 9 Dongge Road,, Nanning, 530000 Guangxi China.
Mei ShiThe First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, NO. 117 Meishan Road, Hefei,, 230031 Anhui, China.
Tingna FuGraduate School of Guangxi University of Traditional Chinese Medicine, No.13 Wuhe AvenueQingxiu District,, Nanning, 530000 , Guangxi China.
Aitao LinThe First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, No. 89 - 9 Dongge Road,, Nanning, 530000 Guangxi China.
Guoliang ZhangThe First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, NO. 117 Meishan Road, Hefei,, 230031 Anhui, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effect of Ursolic acid (UA) on liver cancer cells through cell experiments, in order to inhibit cell proliferation and induce apoptosis.

methodBy adding z-vad-fmk apoptosis inhibitor and ROS inhibitor NAC, CCK8, cell apoptosis, Western blot experiments, mitochondrial membrane potential detection, and ROS flow cytometry were conducted. Compared with the positive drug sorafenib, the inhibitory effect of UA on liver cancer cells and its promotion of apoptosis were studied. The results of CCK8 confirmed that when UA was at its optimal concentration, its inhibitory effect on liver cancer cell viability was greater than that of sorafenib; Through cell apoptosis experiments, it can be observed that under fluorescence microscopy, the fluorescence intensity in the UA+HepG2 group is significantly stronger than that in the sorafenib+HepG2 group; Western blot experiments showed that the relative expression levels of proteins in HepG2 were almost equal between the HepG2+UA and sorafenib+HepG2 groups; through ROS experiments, it was found that when the reactive oxygen species in the HepG2+UA group were lower than those in the sorafenib+HepG2 group, it indicated that the former had a stronger effect of UA on HepG2.

conclusionUA has a significant inhibitory effect on proliferation and promotes apoptosis in liver cancer cells.

Indexed as

AapoptosisCell proliferationHepatocellular carcinoma cellsUrsolic acid

Identifiers

PMID41868709
PMCPMC13000045

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.