ArticleClinical epigenetics2026
The 12 o'clock assay: an optimized dodecaplex droplet digital PCR assay for robust DNA methylation quantification and epigenetic clock-based age-predictions.
Article in Clinical epigenetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Digital PCR in forensic genetics: principles, applications, and challenges.International journal of legal medicine · 2026Review
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16 authors.
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Abstract
DNA methylation quantification in age-prediction models relies on only a few techniques with inherent limitations and biases, reducing the accuracy and reproducibility. We developed a new droplet digital PCR assay format for accurate DNA methylation quantification, based on three LNA-containing hydrolysis probes to detect methylated and unmethylated alleles. Using a 12-plex assay we simultaneously quantified methylation at six CpGs (ASPA, C1orf132, CCDC102B, EDARADD, ELOVL2, FHL2) in 351 blood samples (age range: 0-95 years), showing strong correlations with age (|r|> 0.83). Several age-prediction models were developed, achieving high performance on testing sets (R
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