Evidence map›Paper›PMID 41879849›Full record

ArticleCurrent microbiology2026

An Enzymatic Recombinase Amplification Coupled Lateral Flow Assay for Rapid, Visual, and Cost-Effective Detection of Bordetella pertussis.

Piaoting Wen, Jian Liu, Mingqi Jiao, Hangxi Liu, Mei Lin, Sheping Zhang, Jun He, Ziyi Li, Changhui Xiao, Zhongliang Deng

Abstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Current microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Piaoting Wen *Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Jian Liu *Department of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Mingqi JiaoDepartment of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Hangxi LiuDepartment of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Mei LinSchool of Medical Laboratory, Hunan University of Medicine, Huaihua, 418000, China.
Sheping ZhangClinical Laboratory, The First Affiliated Hospital of Hunan University of Medicine, Huaihua, 418000, China.
Jun HeDepartment of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Ziyi LiDepartment of Public Health Laboratory Sciences, College of Public Health, Hengyang Medical School, University of South China, Hengyang, 421001, China.
Changhui XiaoThe First Affiliated Hospital of University of South China, Hengyang Medical School, University of South China, Hengyang, 421001, China. 1838363801@qq.com.
Zhongliang DengSchool of Medical Laboratory, Hunan University of Medicine, Huaihua, 418000, China. dzl021015@163.com.

Funding

Foundation of State Key Laboratory of Pathogen and Biosecurity of China SKLPBS2437Hunan Province College Student Innovation Training Program S202510555318
6 · The paper itself

Abstract

Bordetella pertussis, the causative agent of pertussis, is a highly contagious respiratory pathogen. Rapid and reliable diagnostics are essential for early detection and disease control. Here, we developed an enzymatic recombinase amplification–lateral flow dipstick (ERA-LFD) assay targeting the IS1663 gene of B. pertussis. The assay achieves high sensitivity with detection limit of 10 copies/µL, and analytical specificity reached 100%. It was performed at 32 ℃ for 15 min, with visual results obtainable within 20 min. Clinical evaluation of 50 nasopharyngeal swab samples demonstrated a sensitivity of 96.6%, specificity of 100%, negative predictive value of 95.2%, and positive predictive value of 100%, showing excellent agreement with quantitative PCR (Kappa = 0.96). These results indicate that the ERA-LFD assay is a rapid, reliable, and field-deployable tool for B. pertussis on-site detection, particularly suitable for point-of-care testing in resource-limited settings.

Indexed as

Bordetella pertussisNucleic Acid Amplification TechniquesRecombinasesWhooping CoughCost-Benefit AnalysisHumansRapid Diagnostic TestsSensitivity and SpecificityRecombinases

Identifiers

PMID41879849

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.