Evidence map›Paper›PMID 41892266›Full record

ArticleBiology2026

Effect of Hydroxyapatite Nanoparticles on the Ultrastructure, Developmental Competence, and Expression of

Xiao-Xia Li, Shi-Yu Zhang, Jun Wang, Yi-Hang Wang, Jia-Hao Zhang, Shi-Han Zhao, Ping-Hua Cao, Yu-Mei Liu, Chen Zhou, Zhen Zhang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xiao-Xia LiCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.ORCID 0000-0001-5580-6261
Shi-Yu ZhangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Jun WangSchool of Information Engineering, Henan University of Science and Technology, Luoyang 471023, China.ORCID 0000-0001-7837-422X
Yi-Hang WangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Jia-Hao ZhangCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Shi-Han ZhaoCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Ping-Hua CaoCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Yu-Mei LiuCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Chen ZhouCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.ORCID 0009-0002-5334-4264
Zhen ZhangHenan Seed Industry Development Center, Zhengzhou 450003, China.
Qiao-Ting ShiInstitute of Animal Husbandry, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Waleid Mohamed El-Sayed ShakweerAgricultural and Biological Research Institute, National Research Centre, Dokki 12622, Egypt.ORCID 0000-0002-5177-2743
Ibrahim Mohamed El-Sayed ShakweerAgricultural and Biological Research Institute, National Research Centre, Dokki 12622, Egypt.ORCID 0009-0005-8210-8668
Zhi-Qian XuCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To improve the vitrification efficiency of bovine germinal vesicle (GV) oocytes, the use of hydroxyapatite (HA) nanoparticles as a novel cryopreservation additive represents a promising approach. This study aimed to investigate the effects of HA nanoparticles and permeable cryoprotective agents (CPAs) on the ultrastructure, developmental competence, and gene expression of bovine GV oocytes following vitrification. Oocytes were vitrified in vitrification solutions containing HA nanoparticles of different sizes (20, 40, or 60 nm) and concentrations (0.01%, 0.05%, or 0.1%) to determine the optimal conditions based on survival rate, mitochondrial membrane potential (MMP) level, and developmental competence. Subsequently, the synergistic effects of HA nanoparticles and permeable CPAs (VS: 20% EG + 20% DMSO; VS1: 17.5% EG + 17.5% DMSO) were further evaluated. The optimal treatment (40 nm 0.05% HA nanoparticles) significantly increased MMP level, and improved developmental competence compared with the vitrified control group (

Indexed as

bovinecryoprotective agentsdevelopmental competencegene expressiongerminal vesicle oocyteshydroxyapatite nanoparticlesultrastructurevitrification

Identifiers

PMID41892266
PMCPMC13023943

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.